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TranscriptomicsGlobal absolute quantification reveals tight regulation of protein expression in single Xenopus eggs
Recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells. In contrast, single cell proteomics analyses have been restricted to targeted analyses, for example using flow cytometry with GFP fusions or mass cytometry. Here, we performed glob...
ORGANISM(S): Xenopus laevis 
2014-08-04 | E-GEOD-56586 | biostudies-arrayexpress
ProteomicsAbsolute proteome quantification in the gas-fermenting acetogen Clostridium autoethanogenum
Microbes that can recycle one-carbon (C1) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO2 and CO. Understanding proteome allocation is important for metabolic engineering ...
ORGANISM(S): Clostridium autoethanogenum 
2022-05-22 | PXD025732 | Pride
Absolute quantification  acetogen  gas fermentation  data-independent acquisition  dia 
ProteomicsMedian Based Absolute Quantification using Fully Unlabelled Generic Internal Standard
By reporting molar abundances of proteins, absolute quantification determines their stoichiometry in complexes, pathways or networks and also relates them to abundances of non-protein biomolecules. Typically, absolute quantification relies either on protein- specific isotopically labelled peptide st...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2021-11-30 | PXD026886 | Pride
Absolute quantification 
ProteomicsAbsolute quantification of budding yeast proteome with PCS method
Absolute quantification of proteome is one of the most important tasks in proteomic research. The aim in this analysis is providing proof-of-principle of our originally developed strategy for absolute quantification of multiple proteins present at various concentrations in a cell, using stable isoto...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-10-06 | PXD003084 | Pride
Absolute quantification Budding yeast Peptide-concatenated standard (pcs) 
ProteomicsThe MS data for accuracy evaluation of absolute quantification algorithms
The DDA and DIA data for accuracy evaluation of absolute quantification algorithms.
ORGANISM(S): Mus Musculus Saccharomyces Cerevisiae 
2018-05-08 | PXD009719 |
ProteomicsGlobal absolute quantification reveals tight regulation of protein expression in single Xenopus eggs
While recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells, single cell proteomics has thus far been restricted to targeted studies. Here, we perform global absolute protein quantification of single Xenopus laevis eggs using mass spec...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2014-07-23 | PXD000902 | Pride
Global absolute quantification reveals tight regulation of protein expression in single xenopus eggs 
ProteomicsLabel-Free Absolute Protein Quantification with Data-Independent Acquisition
Label-free absolute quantitative proteomics is commonly used for absolute quantification of the proteome or specific proteins of interest in various biological samples. Current label-free absolute protein quantification (APQ) methods determine MS1 intensities, MS2 spectral counts or intensities to a...
ORGANISM(S): Homo sapiens (Human) 
2019-03-20 | PXD010912 | Pride
Human Dia S9 Absolute protein quantification Microsome Liver Dda 
ProteomicsAbsolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation
Gene transcription in eukaryotes is regulated through dynamic interactions of a variety of different proteins with DNA in the context of chromatin. Here, we used mass spectrometry for absolute quantification of the nuclear proteome during two stages of Drosophila embryogenesis. Before proteomics ana...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-02-01 | PXD015214 | Pride
Absolute proteome quantification/ histone modification quantification Prc2 H3k27 methylation Drosophila 
UnknownMEERCAT: Multiplexed Efficient Cell Free Expression of Recombinant QconCATs For Large Scale Absolute Proteome Quantification.
Not available
S-EPMC5724179 | biostudies-literature
ProteomicsSelection of reference peptides for absolute quantification of budding yeast proteome
Absolute quantification of proteome is one of the most important tasks in proteomic research. The aim in this analysis is selection of reference tryptic peptides used for stable isotope-labeled standard, based on their spectral peak intensities. Approximate abundance is also calculated by label-free...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-10-06 | PXD003085 | Pride
Absolute quantification Budding yeast Peptide-concatenated standard (pcs) 
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