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To identify the transcripts fractionated into microsome fraction in ribosome-independent manner, we isolate rough microsome fraction by sucrose density gradient ultracengrifugation, then the rough microsome fraction is centrifugated following treatment with puromycine and EDTA in high-salt buffer to...
ORGANISM(S): Drosophila melanogaster 
2016-12-25 | GSE85768 | GEO
Membrane repair machinery is a unique process to maintain cell integrity. In the early step of repair process, TRIM72 facilitates the patch membranes to damaged sites for resealing membrane and protects cells from acute membrane injury. The TRIM72 is an E3 ubiquitin ligase belonging to tripartite mo...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD024978 | Pride
Transcripts enriched in microsome fraction in ribosome-independent manner
Comprehensive proteomic analysis for human liver microsome and human liver cytosol from 25 individual donors focusing on drug metabolising enzymes with relative quantification with TMTpro.
ORGANISM(S): Homo Sapiens (human) 
A pyrethroid-resistant strain of Culex quinquefasciatus, JPal-per, exhibits 2500-fold greater larval resistance to permethrin than the insecticide-susceptible strain Ogasawara. An increased microsome monooxygenase metabolism is involved in the resistance mechanism. Microarray analysis revealed alter...
ORGANISM(S): Culex quinquefasciatus 
Cross-linking mass spectrometry data of synaptosome and microsome fractions of mouse cerebellum and hippocampus.
ORGANISM(S): Mus musculus (Mouse) 
2020-12-03 | PXD010317 | Pride
Label-free absolute quantitative proteomics is commonly used for absolute quantification of the proteome or specific proteins of interest in various biological samples. Current label-free absolute protein quantification (APQ) methods determine MS1 intensities, MS2 spectral counts or intensities to a...
ORGANISM(S): Homo sapiens (Human) 
2019-03-20 | PXD010912 | Pride
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