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MetabolomicsMechanism of Hepatotoxicity Induced by Ethanol Extract of Emilia sonchifolia (L.) DC Revealed by Proteomics and Metabolomics

Objective: Hepatotoxicity has been reported for Emilia sonchifolia (L.) DC (Emilia sonchifolia). The plant material is typically prepared using two extraction methods for practical application: water extraction and ethanol extraction. However, our previous research only investigated its wate...

2025-10-30 | MTBLS13237 | MetaboLights
MetabolomicsRedox proteomics reveal a role for peroxiredoxinylation in stress protection.

The redox state of proteins is essential for their function and guarantees cell fitness. Peroxiredoxins protect cells against oxidative stress, maintain redox homeostasis, act as chaperones, and transmit hydrogen peroxide signals to redox regulators. Despite the profound structural and functional...

2025-07-17 | MTBLS11979 | MetaboLights
TranscriptomicsEstimating accuracy of absolute gene expression measurement by RNA-Seq and microarrays with proteomics
Microarrays revolutionized biological research by enabling gene expression comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute expression level accurately. At present, high throughput sequencing is emerging as an alternative methodology for transcriptome studies...
ORGANISM(S): Homo sapiens 
2010-04-08 | E-GEOD-13744 | biostudies-arrayexpress
TranscriptomicsGlobal absolute quantification reveals tight regulation of protein expression in single Xenopus eggs
Recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells. In contrast, single cell proteomics analyses have been restricted to targeted analyses, for example using flow cytometry with GFP fusions or mass cytometry. Here, we performed glob...
ORGANISM(S): Xenopus laevis 
2014-08-04 | E-GEOD-56586 | biostudies-arrayexpress
TranscriptomicsHeart-muscle expression ratios in pig obtained by 454-sequencing, iTRAQ-based proteomics and cDNA microarrays
The recent development within high-throughput technologies for expression profiling has allowed for parallel analysis of transcriptomes and proteomes in biological systems such as comparative analysis of transcript and protein levels of tissue regulated genes. Until now, such studies of have only in...
ORGANISM(S): Sus scrofa 
2009-01-22 | E-GEOD-10122 | biostudies-arrayexpress
ProteomicsMembrane modulation of super-secreting “midiBacillus” expressing the major Staphylococcus aureus antigen – a mass-spectrometry based absolute quantification approach.
Bacillus subtilis is a Gram-positive bacterium considered as a “cell-factory” for industrial enzymes and biopharmaceuticals. Given the commercial advantage of this organism, researchers have been working towards the improvement of this organism as a producer of secreted proteins. Nonetheless, there ...
ORGANISM(S): Bacillus subtilis subsp. subtilis 
2020-03-20 | PXD015496 | Pride
Srm Membrane proteins Heterologous protein production Proteomics Absolute quantification 
TranscriptomicsEstimating accuracy of absolute gene expression measurement by RNA-Seq and microarrays with proteomics
Microarrays revolutionized biological research by enabling gene expression comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute expression level accurately. At present, high throughput sequencing is emerging as an alternative methodology for transcriptome studies...
ORGANISM(S): Homo sapiens 
2009-10-08 | GSE13744 | GEO
ProteomicsAriadne’s Thread in the Analytical Labyrinth of Membrane Proteins – Integration of Targeted and Shotgun Proteomics for Global Absolute Quantification of Membrane Proteins
The field of systems biology has been rapidly increasing in the past decade. However, the data produced by “omics” approaches is lagging behind the requirements of this field, especially when it comes to absolute abundances of membrane proteins. In the present study, a novel approach for large-scale...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2019-08-26 | PXD014272 | Pride
Shotgun-ms Absolute quantification Targeted-ms Snap-tag Bacillus subtilis Membrane proteins 
ProteomicsGlobal absolute quantification reveals tight regulation of protein expression in single Xenopus eggs
While recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells, single cell proteomics has thus far been restricted to targeted studies. Here, we perform global absolute protein quantification of single Xenopus laevis eggs using mass spec...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2014-07-23 | PXD000902 | Pride
Global absolute quantification reveals tight regulation of protein expression in single xenopus eggs 
UnknownSysQuan: Repurposing SILAC Mice for the Cost-Effective Absolute Quantitation of the Human Proteome.
Not available
S-EPMC12143667 | biostudies-literature
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