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ProteomicsAS precipitation and protein A affinity chromatography for abundant plasma protein depletion
Plasma is a highly valuable resource for biomarker research since it is easy obtainable and contains a high amount of information on patient health status. Although advancements in the field of proteomics enabled analysis of the plasma proteome, identification of low abundant proteins remains challe...
ORGANISM(S): Mus musculus (Mouse) 
2018-05-08 | PXD008969 | Pride
Plasma Mouse Abundant protein depletion 
ProteomicsAffinity capture enrichment versus Affinity depletion: A comparison of strategies for increasing coverage of low abundant human plasma proteins
present study, we evaluated four small molecule affinity-based probes based on agarose-immobilized benzamidine (ABA), O-Phospho-L-Tyrosine (pTYR), 8-Amino-hexyl-cAMP (cAMP), or 8-Amino-hexyl-ATP (ATP) for their ability to remove high-abundant proteins such as serum albumin from plasma samples thereb...
ORGANISM(S): Homo sapiens (Human) 
2020-08-27 | PXD020727 | Pride
Affinity enrichment Depletion Human plasma 
OtherDigestion and depletion of abundant proteins improves proteomic coverage.
Data from Yates lab web site. Experiment: yeast control replicate 2, file: 120211_Yeast_Control2_Step02.mzml. Published as part of Nat Methods. 2013 Jan;10(1):54-6 9 . From the Abstract: {{i}} We exploited the abundance-dependent Michaelis-Menten kinetics of trypsin digestion to selectively digest ...
ORGANISM(S): Yeast 
GPM32320001404 | GPMDB
ProteomicsEvaluation of depletion of abundant plasma proteins for proteomics characterization of EVs
In this study, we aimed to investigate if the removal of abundant plasma proteins prior to EV isolation could improve plasma-derived EV characterization by LC-MS/MS by improving the proteome coverage. Plasma depletion was performed using single-use spin columns prior to SEC-based EV isolation. After...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD039240 | Pride
Ev Plasma depletion Dia Extracellular vesicles Lc-ms/ms 
ProteomicsiEvaluation of spin columns for human plasma depletion to facilitate MS-based proteomics analysis of SARS-cov-2 positive plasman columns for human plasma depletion to facilitate MS-based proteomics analysis of SARS-cov-2 positive plasma
the benefit on the number of identified proteins is well documented and the depletion step remains widely used in in-depth proteomics. Recently, single-use depletion spin columns have been developed (i.e High Select™ Top14 Abundant Protein Depletion Mini Spin Columns, Thermo) which greatly reduces ...
ORGANISM(S): Homo sapiens (Human) Macaca mulatta (Rhesus macaque) 
2021-07-30 | PXD023148 | Pride
Proteomics Mass spectrometry Plasma Depletion Sars-cov-2 Human Spin-column 
ProteomicsRabbit serum proteome analysis by various Serum Abundant Protein Depletion Kits
Rabbit serum proteome was analyzed by various Serum Abundant Protein Depletion Kits.
ORGANISM(S): Oryctolagus Cuniculus (ncbitaxon:9986) 
2023-02-09 | MSV000091233 | MassIVE
UnknownDepletion of Highly Abundant Protein Species from Biosamples by the Use of a Branched Silicon Nanopillar On-Chip Platform.
Not available
S-EPMC8592501 | biostudies-literature
ProteomicsPlasma samples processed with depletion and non depletion
Plasma samples processed with no depletion and most-abundant-proteins depletion procedures according to standard in-solution and single-pot, solid-phase-enhaced sample preparation (SP3) workflows using guanidyne hydrochloride and SDS lysis buffers for analysis with liquid chromatography mass spectro...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD031919 | Pride
Plasma 
UnknownDigestion and depletion of abundant proteins improves proteomic coverage.
Not available
S-EPMC3531578 | biostudies-literature
UnknownSupramolecular Polymeric Assemblies for the Selective Depletion of Abundant Acidic Proteins in Serum.
Not available
S-EPMC6791357 | biostudies-literature
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