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ProteomicsFe3+-NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment
Protein phosphorylation is a central mechanism of cellular signal transduction in living organisms. Phosphoproteomic studies aim to systematically catalogue, characterize, and comprehend alterations in phosphorylation states across multiple cellular conditions and are often incorporated into global ...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2022-09-02 | PXD031327 | Pride
Automation Phosphoproteome Imac Phosphopeptide enrichment 
ProteomicsSpin column-based prefractionation alternatives for Streamlined Tandem Mass Tag (SL-TMT) sample processing
Fractionation is essential to achieving deep proteome coverage for sample multiplexing experiments where currently up to 18 samples can be analyzed concurrently. However, prefractionation (i.e., upstream of LC-MS/MS analysis) with a liquid chromatography system constrains sample processing as only a...
ORGANISM(S): Homo sapiens (Human) 
2023-12-28 | PXD038383 | Pride
Faims Tmtpro Hprp Isobaric tagging Sax 
ProteomicsSuspension TRAPping filter (sTRAP) sample preparation for quantitative proteomics in the low µg input range using a plasmid DNA micro-spin column: analysis of the hippocampus from the 5xFAD Alzheimer’s Disease mouse model
Suspension TRAPping filter (sTRAP) is an attractive sample preparation method for proteomics study. Here, we demonstrated the use of a low-cost Plasmid DNA spin column for sTRAP. We first evaluated, the reproducibility of this protocol and the low CoV using 4 replicates of 5-10 µg of a synapse enric...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-23 | PXD041045 | Pride
Alzheimer’s disease Proteomics 5xfad mouse model Micro-spin column Strap 
UnknownA spin column-free approach to sodium hydroxide-based glycan permethylation.
Not available
S-EPMC5973876 | biostudies-literature
UnknownFe3+-NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment.
Not available
S-EPMC9195054 | biostudies-literature
UnknownSpin column-based peptide fractionation alternatives for streamlined tandem mass tag (SL-TMT) sample processing.
Not available
S-EPMC9990130 | biostudies-literature
UnknownCharacterization of RNA from Exosomes and Other Extracellular Vesicles Isolated by a Novel Spin Column-Based Method.
Not available
S-EPMC4552735 | biostudies-literature
TranscriptomicsExpression profiles in Blast Bank samples with AML/RAEB
CD133-positive immature blasts were purified from bone marrow of the patients. Total RNA was extracted by RNeasy mini spin column (Qiagen, Inc).
ORGANISM(S): Homo sapiens 
2006-05-25 | GSE1427 | GEO
UnknownA Rapid Spin Column-Based Method to Enrich Pathogen Transcripts from Eukaryotic Host Cells Prior to Sequencing.
Not available
S-EPMC5176299 | biostudies-literature
UnknownSuspension TRAPping Filter (sTRAP) Sample Preparation for Quantitative Proteomics in the Low µg Input Range Using a Plasmid DNA Micro-Spin Column: Analysis of the Hippocampus from the 5xFAD Alzheimer's Disease Mouse Model.
Not available
S-EPMC10177283 | biostudies-literature
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