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ProteomicsPurification of Human β and γ Actin from Budding Yeast
To facilitate biochemical studies of human cytoplasmic actins, Saccharomyces cerevisiaestrains were developed that express either human β-actin or γ-actin as their sole actin. Actins purified from these strains have biochemical properties similar to mammalian skeletal muscle α-actin, but display di...
ORGANISM(S): Oryctolagus sp. 'Rabbit_OD' Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2023-04-24 | PXD040174 | Pride
Β-actin Cytoplasmic actin Non-muscle actin Γ-actin 
GenomicsActin binding proteins expression levels when modifying the actin sequence
Actin binding proteins expression levels when modifying the actin sequence
PRJNA782536 | ENA
TranscriptomicsA putative network of actin-associated cytoskeletal proteins in glomerular podocytes defined by co-purified mRNAs
Identification of a putative network of actin-associated cytoskeletal proteins in glomerular podocytes defined by co-purified mRNAs. An experimental purification in which a presumed RNA binding protein was used for an RNA immunoprecipitation and microarray analysis (RIP-CHIP). Total cell extracts w...
ORGANISM(S): Mus musculus 
2009-08-01 | E-GEOD-16942 | biostudies-arrayexpress
ProteomicsKLF2-induced actin shear fibers control both alignment to flow and JNK signaling in vascular endothelium
The shear stress-induced transcription factor Krüppel-like factor 2 (KLF2) confers anti-inflammatory properties to endothelial cells through inhibition of activator protein 1, presumably by interfering with MAPK cascades. To gain insight into the regulation of these cascades by KLF2, we used antibo...
ORGANISM(S): Homo sapiens 
2010-06-25 | E-GEOD-19412 | biostudies-arrayexpress
TranscriptomicsForm and function of actin impacts actin health and aging.RNAseq
The actin cytoskeleton is a highly conserved structural network that supports diverse cellular processes, but its contribution to organismal aging is not well understood. This project investigates how genetic and pharmacological perturbations of actin and actin-binding proteins influence global gene...
ORGANISM(S): Caenorhabditis elegans 
2026-05-11 | E-MTAB-16193 | biostudies-arrayexpress
TranscriptomicsExpression in arp6 and swr1 deletion mutants
Actin-related proteins are ubiquitous components of chromatin remodelers, and are conserved from yeast to man. We have examined the role of the budding yeast actin-related protein Arp6 in gene expression, both as a component of the SWR1 complex (SWR-C) and in its absence. We mapped Arp6-binding site...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-01 | E-GEOD-17780 | biostudies-arrayexpress
GenomicsActin associates with actively elongating genes
Actin associates with actively elongating genes
PRJNA1000942 | ENA
GenomicsForm and function of actin impacts actin health and aging.RNAseq
Form and function of actin impacts actin health and aging.RNAseq
PRJEB104007 | ENA
TranscriptomicsComparative transcriptome analysis of DFAT cells after the treatment with Y-27632 and the transfection of Mkl1 siRNA
Cellular differentiation is regulated through activation and repression of defined transcription factors. A hallmark of differentiation is a pronounced change in cell shape, which is determined by dynamics of the actin cytoskeleton. In de-differentiated fat (DFAT) cells and 3T3-L1 cells, we showed t...
ORGANISM(S): Mus musculus 
2014-03-28 | E-GEOD-52334 | biostudies-arrayexpress
TranscriptomicsForm and function of actin impacts actin health and aging - snRNAseq
The actin cytoskeleton is a highly conserved structural network that supports diverse cellular processes, but its contribution to organismal aging is not well understood. This project investigates how genetic and pharmacological perturbations of actin and actin-binding proteins influence global gene...
ORGANISM(S): Caenorhabditis elegans 
2026-05-07 | E-MTAB-16195 | biostudies-arrayexpress
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