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ProteomicsDevelopment of Substrate-Directed Activity-Based Probes via Disulfide-Trapping for Site-Specific Interrogation of Nucleosomal Deubiquitination
Activity-based ubiquitin probes (Ub-ABPs) have been instrumental in interrogating deubiquitinases (DUBs), yet conventional Ub-ABPs lack the substrate-specific and site-specific contextual cues required to investigate nucleosomal DUBs, whose activity is tightly regulated by the chromatin environments...
ORGANISM(S): Homo Sapiens 
2025-12-30 | PXD072417 |
ProteomicsDiscovery and mechanism of K63-linkage-directed deubiquitinase activity in USP53
Ubiquitin-specific proteases (USPs) are the largest class of human deubiquitinases (DUBs) and comprise its phylogenetically most distant members USP53 and USP54, which are annotated as catalytically inactive pseudo-enzymes. Conspicuously, mutations in the USP domain of USP53 cause progressive famili...
ORGANISM(S): Homo sapiens (Human) 
2024-11-11 | PXD054748 | Pride
Ubiquitin chain length Dubs Ubiquitin signaling K63 linkage X-ray crystallography Cholestasis Deubiquitinating enzymes Protease mechanism Ubiquitin probes Ubiquitin Polyubiquitin specificity Usp54 Usp53 
ProteomicsADAM17 cytoplasmic domain modulates Thioredoxin-1
We have previously shown that Thioredoxin-1 (Trx-1) binds to the cytoplasmic domain of ADAM17 (ADAM17cyto), A Disintegrin And Metalloprotease 17, and that the destabilization in their interface of interaction favors the Trx-1 dimeric and inactive state. These studies opened the question of whether A...
ORGANISM(S): Homo Sapiens 
2021-03-12 | PXD020016 | panorama
UnknownTargeting proline in (phospho)proteomics.
Not available
S-EPMC7496877 | biostudies-literature
UnknownDiscovery and mechanism of K63-linkage-directed deubiquitinase activity in USP53.
Not available
S-EPMC12037411 | biostudies-literature
ProteomicsActivity-based proteomic profiling (ABPP) employing a serine hydrolase-directed probe to identify changes in activity profiles of lipid hydrolase in lung tumors
Lung tumors, as well as normal tumor-adjacent (NTA) tissue of non-small cell lung cancer (NSCLC) patients, were collected and subjected to labeling with a serine hydrolase-directed activity-based probe (ABP) immediately after excision. Labeling with the serine hydrolase-directed ABP allows for the d...
ORGANISM(S): Homo sapiens (Human) 
2023-11-27 | PXD046934 | Pride
Abpp Lipid hydrolysis Serine hydrolase Nsclc Lipid hydrolase Lung tumor Activity-based proteomic profiling Lipase 
ProteomicsDual-Probe Activity-Based Protein Profiling Reveals Site-Specific Binding Dynamics of EGFR-Directed Drugs
Comparative, dose-dependent analysis of interactions between small molecule drugs and their targets, as well as off-targets, in complex proteomes is crucial for selecting optimal drug candidates. The affinity of small molecules for targeted proteins is largely dictated by interactions between amino ...
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD045864 | Pride
Chemical proteomics  activity-based protein profiling  site-specific  concentration-dependent analysis  dual-probe comparison. 
UnknownHost-Directed Antiviral Activity of SB2960 Through Selective Induction and Remodeling of Stress Granules.
Not available
S-EPMC12948273 | biostudies-literature
UnknownIdentification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification.
Not available
S-EPMC4553411 | biostudies-literature
UnknownA biotin switch-based proteomics approach identifies 14-3-3ζ as a target of Sirt1 in the metabolic regulation of caspase-2.
Not available
S-EPMC3417139 | biostudies-literature
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