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ProteomicsAnalysis of insulin-regulated phosphorylation of AFF4 with Akti or S6ki and S6K invitro kinase
Aff4 was recently identified as an insulin-regulated phosphoprotein. To investigate upstream kinases, we analysed immunoprecipitated Aff4 treated with our without insulin and treated with either an Akti or a P70S6Ki. These results showed that AFF4 is dependent on P70S6K and therefore an in vitro kin...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-16 | PXD054247 | Pride
Akt P70s6k Phosphorylation Aff4 
ProteomicsAffinity purification – mass spectrometry of AFF4 WT or S829/S831/3/5/8A mutant
The S831 phosphosite on AFF4 lies within a disordered region adjacent to the ENL/AF9 binding site. We therefore hypothesized that phosphorylation may regulate protein:protein interactions within the Super Elongation Complex. To investigate this, HEK293T cells expressing RFP negative control, FLAG-ta...
ORGANISM(S): Homo sapiens (Human) 
2025-12-16 | PXD054250 | Pride
Affinity purification Aff4 Interactome 
TranscriptomicsGene target specificity of the Super Elongation Complex (SEC) family: How HIV-1 Tat employs selected SEC members to activate viral transcription
The AF4/FMR2 proteins AFF1 and AFF4 act as a scaffold to assemble the Super Elongation Complex (SEC) that strongly activates transcriptional elongation of HIV-1 and cellular genes. Although they can dimerize, it is unclear whether the dimers exist and function within a SEC in vivo. Furthermore, it i...
ORGANISM(S): Homo sapiens 
2015-05-20 | E-GEOD-69021 | biostudies-arrayexpress
TranscriptomicsThe SEC family of RNA Polymerase II elongation factors: gene target specificity and transcriptional output
The elongation stage of transcription is a highly regulated in metazoan. We previously purified the AFF1/AFF4-containing Super Elongation Complex (SEC) as a major regulator of development and cancer pathogenesis. Here, we report the biochemical isolation of SEC-like 2 (SEC-L2) and SEC-like 3 (SEC-L3...
ORGANISM(S): Homo sapiens 
2012-05-01 | E-GEOD-34097 | biostudies-arrayexpress
GenomicsAFF4 regulates cellular adipogenic differentiation via targeting autophagy
AFF4 regulates cellular adipogenic differentiation via targeting autophagy
PRJNA810021 | ENA
TranscriptomicsTranscriptome characterization of CHOPS syndrome, a novel genetic disorder caused by gain-of-function mutations of AFF4
AFF4 is a component of super elongation complex (SEC), which plays an important role in mobilizing paused RNA polymerase II at gene promoter regions. Using exome sequenging, we have identified a novel genetic disorder caused by missense mutations in AFF4. We propose CHOPS syndrome as a name for th...
ORGANISM(S): Homo sapiens 
2015-01-27 | E-GEOD-64031 | biostudies-arrayexpress
UnknownAFF4 regulates cellular adipogenic differentiation via targeting autophagy.
Not available
S-EPMC9534390 | biostudies-literature
TranscriptomicsAFF4 regulates cellular adipogenic differentiation via targeting autophagy
AFF4 was discovered as a scaffold protein of Super Elongation Complex (SEC) and exhibites an essential function on osteogenesis, tumorigenesis and odontogenesis. However, the adipogenic biological functions of AFF4 need further explorations. Here, we demonstrate that knockdown of AFF4 inhibits adipo...
ORGANISM(S): Homo sapiens 
2023-02-28 | GSE197354 | GEO
GenomicsEffects of FOXN1 or AFF4 deficiency on gene expression in the skin
Effects of FOXN1 or AFF4 deficiency on gene expression in the skin
PRJNA638718 | ENA
UnknownPI3K/ c-Myc/AFF4 axis promotes pancreatic tumorigenesis through fueling nucleotide metabolism.
Not available
S-EPMC10092763 | biostudies-literature
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