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ProteomicsMceC affinity purification and interactome analysis
Anti-FLAG affinty purification of mitochondria isolated from tetracycline inducible HEK293 cells expressing MceC-3XFLAG or control (EV) cells. Equal amounts of mitochondrial protein were solubilised and anti-FLAG immunoprecipitation performed.
ORGANISM(S): Homo sapiens (Human) Coxiella burnetii 
2020-11-12 | PXD019339 | Pride
Effector protein Affinty purification Coxiella 
ProteomicsMceC affinity purification and interactome analysis
Anti-FLAG affinty purification of mitochondria isolated from tetracycline inducible HEK293 cells expressing MceC-3XFLAG or control (EV) cells. Equal amounts of mitochondrial protein were solubilised and anti-FLAG immunoprecipitation performed.
ORGANISM(S): Homo sapiens (Human) Coxiella burnetii 
2020-11-12 | PXD019417 | Pride
Coxiella burnetii Effector protein Mitochondria 
TranscriptomicsMicroglia-astrocyte crosstalk regulates synapse remodeling via Wnt signaling
Unilateral whisker lesioning at age P4 leads to removal of thalamocortical synapses in layer IV of the barrel cortex over the course of the next 6 days. Synapse removal is mediated by microglia and induced by CX3CL1-CX3CR1 signaling between neurons and microglia. Downstream of CX3CL1-CX3CR1 activati...
ORGANISM(S): Mus musculus 
2025-08-07 | GSE252628 | GEO
ProteomicsCharacterisation of two distinct Nucleosome Remodelling and Deacetylase Complex (NuRD) assemblies in embryonic stem cells.
Pluripotency and self-renewal, the defining properties of embryonic stem cells, are brought about by transcriptional programs involving an intricate network of transcription factors and chromatin remodelling complexes. The Nucleosome Remodelling and Deacetylase (NuRD) complex plays a crucial and dyn...
ORGANISM(S): Mus musculus (Mouse) 
2016-01-05 | PXD002452 | Pride
Affinty purification Deacetylase complex Mouse Embryonic stem cells Nucleosome remodelling Nurd Lc-ms/ms Blue native 
ProteomicsChemoproteomic target deconvolution of Lipoic acid reveals Histone Deacetylases as the main targets
Here we set out to identify protein targets of Lipoic acid and Lipoamide by chemical proteomics approaches. We generated Lipoamide-based affinity matrices to pulldown target proteins and quantify them via bottom-up proteomics. Use of the same affinity matrix in dose dependent compeition assays allow...
ORGANISM(S): Homo sapiens (Human) 
2026-06-02 | PXD036810 | Pride
Lc-msms Drug target deconvolution Cancer cell lines 
GenomicsMicroglia-astrocyte crosstalk regulates synapse remodeling via Wnt signaling
Microglia-astrocyte crosstalk regulates synapse remodeling via Wnt signaling
PRJNA1061535 | ENA
ProteomicseXL-MS: An enhanced Cross-Linking Mass Spectrometry Workflow to Study Protein Complexes.
The analysis of proteins and protein complexes by cross-linking mass spectrometry (XL-MS) has expanded in the last dec-ade. However, mostly used approaches suffer important limitations in term of efficiency and sensitivity. We describe here a new workflow based on the advanced use of the trifunction...
ORGANISM(S): Oryctolagus cuniculus (Rabbit) 
2021-08-11 | PXD010422 | Pride
Creatine kinase Mass spectrometry Cross-link Monoavidin biotin affinty enrichment Click chemistry 
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