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ProteomicsUse of data-independent acquisition mass spectrometry to identify an objective serum indicator of the need for osteoporotic therapeutic intervention
Osteoporosis is characterized by weakened bone microstructure and loss of bone mass. Current diagnostic criteria for osteoporosis are based on the T-score, which is a measure of bone mineral density. However, osteoporotic fragility fractures can occur regardless of the T-score, underscoring the need...
ORGANISM(S): Homo Sapiens (human) 
2024-04-10 | PXD041546 | JPOST Repository
ProteomicsComprehensive human serum spectral library
For a comprehensive analysis of the human serum proteome, we constructed a spectral library for DIA-MS. Our spectral library contained information on 20,303 peptides derived from 1,597 human serum proteins. We believe that the constructed spectral library will be useful for proteomic analysis of hum...
ORGANISM(S): Homo Sapiens (human) 
2024-04-10 | PXD041558 | JPOST Repository
ProteomicsChanges in the astronaut serum proteome during prolonged spaceflight
The molecular mechanisms associated with spaceflight-induced biological adaptations that may affect many healthy tissue functions remain poorly understood. In this study, we analyzed temporal changes in the serum proteome of six astronauts during prolonged spaceflight missions using quantitative com...
ORGANISM(S): Homo Sapiens (human) 
2024-01-22 | PXD044609 | JPOST Repository
ProteomicsEGF-induced regulation of human Gamma-enolase interactome
EGF-induced regulation of Gamma-enolase protein complexes was analyzed using anti-Gamma-enolase monoclonal antibodies designed to immunoreact with the C-terminus of human Gamma-enolase protein.
ORGANISM(S): Homo Sapiens (human) 
2026-05-17 | PXD070141 | JPOST Repository
ProteomicsTMT-based quantitative proteomic analysis of EGF-treated human cancer cells
We performed a TMT-based quantitative proteomic analysis of EGF-treated human cancer cells in the presence/absence of Torin 1 using advanced RTS platform on Orbitrap Eclipse Tribrid MS system.
ORGANISM(S): Homo Sapiens (human) 
2026-05-17 | PXD064188 | JPOST Repository
ProteomicsSelective Enrichment of Newly Synthesized Proteins using Phos-tag Click Tip Enables Nascent Proteome Analysis in Influenza A Virus Infection
Bioorthogonal non-canonical amino acid tagging (BONCAT)-based approaches enable selective labeling of newly synthesized proteins (NSPs). We developed a workflow termed the “Phos-tag Click Tip” by integrating a phosphorylated variant of bicyclononyne (pBCN) with Phos-tag affinity purification to sele...
ORGANISM(S): Homo Sapiens (human) 
2026-09-09 | PXD059239 | JPOST Repository
ProteomicsThe customized mouse serum spectral library for the DIA-MS
For a comprehensive analysis of the mouse serum proteome, a customized mouse serum spectral library was constructed and utilized for quantitative analysis using DIA-MS. Our spectral library contained information on 1,106 mouse serum proteins. Creation of the spectral library was performed using Spec...
ORGANISM(S): Mus Musculus (mouse) 
2024-03-16 | PXD041889 | JPOST Repository
ProteomicsIdentification of gravity-responsive serum proteins in spaceflight mice using a quantitative proteomic approach with data-independent acquisition mass spectrometry
Physical inactivity associated with gravity unloading, such as microgravity during spaceflight and hindlimb unloading (HU), can cause various physiological changes. In this study, we attempted to identify serum proteins whose levels fluctuated in response to gravity unloading. First, we quantitative...
ORGANISM(S): Mus Musculus (mouse) 
2024-03-16 | PXD041894 | JPOST Repository
ProteomicsCharacterization of phosphopeptide enrichment strategy for mass spectrometry-based proteomic analysis
We attempted to evaluate four strategies for phosphopeptide enrichment, using the titanium dioxide (TiO2) and Phos-tag ligand particles, from in-solution or in-gel digests prior to mass spectrometry-based proteomic analysis.
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2021-12-24 | PXD026295 | JPOST Repository
ProteomicsIdentification of gravity-responsive proteins in mouse soleus muscle using proteomic analysis
This project is “Medical proteome analysis of osteoporosis- and bone mass-related proteins using the Kibo Japanese Experiment Module of International Space Station (ISS)”. Applying proteomic technologies, this research aims to identify proteins involved in space flight-induced osteopenia as well as ...
ORGANISM(S): Mus Musculus (mouse) 
2023-09-19 | PXD041190 | JPOST Repository
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