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ProteomicsDeciphering Diazirine Reaction Mechanism: through Irradiation Modulation for Residue-Specific Distance Restraints in Protein Photo-Crosslinking
The diazirine photoreactive group has been widely used for photo-labeling and photo-cross-linking (PXL) of proteins. Yet, due to the mechanistic complexity of diazirine photo-reaction, site-specific and quantitative analysis of protein PXL data remains challenging. Herein, we have built an in-line p...
ORGANISM(S): Bos taurus (Bovine) 
2024-06-22 | PXD048452 | Pride
Photo cross-linking Multiple reaction monitoring Optical power density Distance restraints Alkyl diazirine 
ProteomicsDesign and evaluation of a cyclobutane diazirine alkyne tag for photoaffinity labeling in cells
Alkyl diazirines are frequently used in photoaffinity labeling to trap and identify small molecule–protein interactions. How-ever, the alkyl diazirine can preferentially label acidic amino acids and acidic protein surfaces in a pH-dependent manner, presumably via a long-lived alkyl diazo intermediat...
ORGANISM(S): Homo sapiens (Human) 
2024-01-04 | PXD035954 | Pride
Cereblon Diazirine Thalidomide Photoaffinity labeling Binding site mapping 
UnknownDesign and Evaluation of a Cyclobutane Diazirine Alkyne Tag for Photoaffinity Labeling in Cells.
Not available
S-EPMC11647570 | biostudies-literature
ProteomicsLabeling preferences of diazirines with protein biomolecules
Diazirines are widely used in photoaffinity labeling (PAL) to trap non-covalent interactions with biomolecules. However, design and interpretation of PAL experiments is challenging without a molecular understanding of the reactivity of diazirines with protein biomolecules. Here, we report a system...
ORGANISM(S): Homo sapiens (Human) 
2022-07-20 | PXD025140 | Pride
Sknsh cells Diazirine Photoaffinity labeling Proteomics Binding sites 
UnknownRecognition of diazirine-modified O-GlcNAc by human O-GlcNAcase.
Not available
S-EPMC4109824 | biostudies-literature
UnknownDissecting diazirine photo-reaction mechanism for protein residue-specific cross-linking and distance mapping.
Not available
S-EPMC11258254 | biostudies-literature
UnknownGenetic Encoding of a Trifunctional Photo-Cross-Linker with a Cleavable Alkyl Ester Moiety.
Not available
S-EPMC12859177 | biostudies-literature
UnknownLabeling Preferences of Diazirines with Protein Biomolecules.
Not available
S-EPMC11647638 | biostudies-literature
UnknownBifunctional Probes Reveal the Rules of Intracellular Ether Lipid Transport.
Not available
S-EPMC12603971 | biostudies-literature
UnknownBoronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies.
Not available
S-EPMC6844280 | biostudies-literature
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