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Metabolomics4-Octyl itaconate as a metabolite derivative inhibits inflammation via alkylation of STING

The Krebs cycle-derived metabolite itaconate, whose production is catalyzed by immune response gene 1 (IRG1), has excellent potential to link immunity and metabolism in activated macrophages through alkylation or competitive inhibition of target proteins. In support of this, our previous study de...

2023-01-15 | MTBLS5877 | MetaboLights
ProteomicsSystematic Evaluation of Protein Reduction and Alkylation reveals Massive Unspecific Side Effects by Iodine-containing Reagents
In this study, we systematically evaluated reduction and alkylation of proteins using three reducing agents (DTT, TCEP, and BME) in combination with four alkylation agents (IAA, IAC, AA, and CAA). We tested these conditions using strong anion exchange (SAX) fractionated in-solution digests and in-ge...
ORGANISM(S): Homo sapiens (Human) 
2017-05-30 | PXD005183 | Pride
Alkylation Proteomics Reduction 
Proteomics‘Shotgun’ Proteome Analyses Do Not Require Alkylation of Cysteine
It is a common belief that reduction of disulfide bridges and alkylation of thiols in proteins are indispensable steps in proteomic sample preparation. Since this chemical procedure is often incomplete and prone to side reactions and can results in various artefacts we reexamined its importance. We ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-21 | PXD014000 | Pride
Thiol alkylation Iodoacetamide Shotgun proteomics 
ProteomicsAlkylation Damage by Lipid Electrophiles Targets Functional Protein Systems
These data correspond to "Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems," a paper from Codreanu, Liebler, et al, published at the journal _Molecular and Cellular Proteomics_ in 2013. Modifications also include: 4-hydroxynonenal (HNE) (UniMod #53), H(16) C(9) O(2), Mono...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2013-11-18 | MSV000078490 | MassIVE
ProteomicsAlkylation Damage by Lipid Electrophiles-- HNE-induced expression in RKO cells
4-hydroxynonenal (HNE) (UniMod 53) (+156.115030 Da on Cys, His, Lys) reduced 4-Hydroxynonenal (UniMod 335(+158.130680 Da on Cys, His, Lys) These data were additions to the set for the paper Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems by Codreanu et al, Mol. Cell. Pro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2013-12-18 | MSV000078512 | MassIVE
ProteomicsDifferential alkylation of PHLPP2
To determine the accessibility of cysteines in purified PHLPP2, we assessed their reactivity with iodoacetamide under native conditions. After denaturing with urea and reducing potential disulfide bridges with TCEP, the remaining free cysteines were modified with methyl methanethiosulfonate. This 2-...
ORGANISM(S): Homo sapiens (Human) 
2025-03-13 | PXD052551 | Pride
Differential alkylation Free thiols 
GenomicsAberrant RNA methylation triggers recruitment of an alkylation repair complex
Aberrant RNA methylation triggers recruitment of an alkylation repair complex
PRJNA663914 | ENA
GenomicsSynthetic construct Homo sapiens clone FLH119406.01L alkB, alkylation repair-like (E. coli) mRNA, partial cds
Synthetic construct Homo sapiens clone FLH119406.01L alkB, alkylation repair-like (E. coli) mRNA, partial cds
PRJEB97886 | ENA
GenomicsSynthetic construct Homo sapiens clone FLH119406.01L alkB, alkylation repair-like (E. coli) mRNA, partial cds
Synthetic construct Homo sapiens clone FLH119406.01L alkB, alkylation repair-like (E. coli) mRNA, partial cds
PRJEB97885 | ENA
GenomicsChromatin regulator HELLS mediates SSB repair and responses to DNA alkylation damage
Chromatin regulator HELLS mediates SSB repair and responses to DNA alkylation damage
PRJNA1195851 | ENA
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