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ProteomicsCIAA: Integrated Proteomics and Structural Modeling for Understanding Cysteine Reactivity with Iodoacetamide Alkyne
This study utilizes cysteine chemoproteomics paired with a high-throughput structure-guided in silico approach towards assessing structural motifs of cysteines that are highly reactive towards iodoacetamide alkyne (IAA).
ORGANISM(S): Homo sapiens (Human) 
2025-10-13 | PXD056064 | Pride
Reactivity Cysteine Chemoproteomics Isotop-abpp 
UnknownIsotopically-Labeled Iodoacetamide-Alkyne Probes for Quantitative Cysteine-Reactivity Profiling.
Not available
S-EPMC5841085 | biostudies-literature
UnknownCIAA: Integrated Proteomics and Structural Modeling for Understanding Cysteine Reactivity with Iodoacetamide Alkyne.
Not available
S-EPMC12503369 | biostudies-literature
UnknownProtective Effects of Fucoidan on Iodoacetamide-Induced Functional Dyspepsia via Modulation of 5-HT Metabolism and Microbiota.
Not available
S-EPMC11989908 | biostudies-literature
ProteomicsHuman proteome derivatized with iodoacetamide and acrylamide
This dataset was generated to parametrize and benchmark software for unbiased discovery of post-translationally modified peptides.
ORGANISM(S): Homo sapiens (Human) 
2021-07-22 | PXD019793 | Pride
Lc-msms iodoacetamide acrylamide modifications ptm orbitrap fusion 
TranscriptomicsRNA-sequencing using SLAM-sequencing protocol of human iPSC-derived lower motor neurons treated with BDNF for 1, 2, 6 h
SLAM-sequencing was performed on i3 LMNs following protocols adapted from Herzog et al. (2017). Three biological replicates of i3 LMNs were treated with 100 µM 4sU (Control) + labelling medium or 100 µM 4sU + BDNF (BDNF condition) labelling medium on Day 7 for 1h, 2h, and 6h. Cells were washed with ...
ORGANISM(S): Homo sapiens 
2025-05-30 | E-MTAB-14537 | biostudies-arrayexpress
ProteomicsDifferential alkylation of PHLPP2
To determine the accessibility of cysteines in purified PHLPP2, we assessed their reactivity with iodoacetamide under native conditions. After denaturing with urea and reducing potential disulfide bridges with TCEP, the remaining free cysteines were modified with methyl methanethiosulfonate. This 2-...
ORGANISM(S): Homo sapiens (Human) 
2025-03-13 | PXD052551 | Pride
Differential alkylation Free thiols 
Proteomics‘Shotgun’ Proteome Analyses Do Not Require Alkylation of Cysteine
It is a common belief that reduction of disulfide bridges and alkylation of thiols in proteins are indispensable steps in proteomic sample preparation. Since this chemical procedure is often incomplete and prone to side reactions and can results in various artefacts we reexamined its importance. We ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-21 | PXD014000 | Pride
Thiol alkylation Iodoacetamide Shotgun proteomics 
TranscriptomicsRNA-seq of metabolically labelled RNA by 4-thiouracil from mouse tissues
To identify cell type-specific RNA without cell sorting, mice expressing UPRT in a specific cell type were exposed to 4-thiouracil to label RNA only in the UPRT-expressing cells. Total RNA was extracted from tissues and treated with iodoacetamide followed by RNA-seq library preparation. The reads ge...
ORGANISM(S): Mus musculus 
2018-07-09 | E-MTAB-6353 | biostudies-arrayexpress
UnknownOxidation of p53 through DNA charge transport involves a network of disulfides within the DNA-binding domain.
Not available
S-EPMC4310631 | biostudies-literature
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