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ProteomicsProteasome-dependent truncation of the negative heterochromatin regulator Epe1 mediates antifungal resistance.
Epe1 histone demethylase restricts H3K9-methylation-dependent heterochromatin, preventing it from spreading over, and silencing, gene-containing regions in fission yeast. External stress induces an adaptive response allowing heterochromatin island formation that confers resistance on surviving wild-...
ORGANISM(S): Schizosaccharomyces pombe 927 
2022-10-13 | PXD030205 | Pride
Fission yeast Epe1 Shotgun proteomics. Anti-fungal resistance H3k9-methylation Proteasome Heterochromatin 
TranscriptomicsHairpin RNA induces secondary small interfering RNA synthesis and silencing in trans in fission yeast
We provide evidence of the generation of Rdp1-mediated secondary siRNAs in vivo in fission yeast. Secondly, we show that the presence of Ago1-associated siRNAs does not guarantee robust silencing. We obtained sequences from gel purified small RNAs of wt and rdp1delta fission yeast cells and FLAG-ago...
ORGANISM(S): Schizosaccharomyces pombe 
2010-02-15 | E-GEOD-20330 | biostudies-arrayexpress
TranscriptomicsFactors that promote H3 chromatin integrity during transcription prevent promiscuous deposition of CENP-A(Cnp1) in fission yeast
Specialized chromatin containing CENP-A nucleosomes instead of H3 nucleosomes is found at all centromeres. However, the mechanisms that specify the locations at which CENP-A chromatin is assembled remain elusive in organisms with regional, epigenetically regulated centromeres. It is known that norma...
ORGANISM(S): Schizosaccharomyces pombe 
2015-02-19 | E-GEOD-66106 | biostudies-arrayexpress
TranscriptomicsA DNA polymerase ? accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast
Specialized chromatin exists at centromeres and must be precisely transmitted during DNA replication. The mechanisms involved in the propagation of these structures remain elusive. Fission yeast centromeres are composed of two chromatin domains: the central CENP-ACnp1 kinetochore domain and flanking...
ORGANISM(S): Schizosaccharomyces pombe 
2008-07-22 | E-GEOD-11102 | biostudies-arrayexpress
TranscriptomicsTelomeric repeats facilitate CENP-A incorporation via telomere binding proteins
The histone H3 variant, CENP-ACnp1, is normally assembled upon canonical centromeric sequences, but there is no apparent obligate coupling of sequence and assembly, suggesting that centromere location can be epigenetically determined. To explore the tolerances and constraints on CENP-ACnp1 depositio...
ORGANISM(S): Schizosaccharomyces pombe 
2013-08-22 | E-GEOD-46427 | biostudies-arrayexpress
TranscriptomicsA histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice.
Wildtype and set2delta strains, with samples taken at t=0 and following 30 minutes treatment with 5 µg/ml bleomycin.
ORGANISM(S): Schizosaccharomyces pombe 
2014-06-09 | E-MTAB-2549 | biostudies-arrayexpress
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