The 5’ single-cell library was prepared strictly following the manufacturer's instructions (User Guide CG000733, 10x Genomics) using the Chromium GEM-X Single Cell 5' Kit v3 (cat# PN-1000701).
Streptococcus suis is a zoonotic pathogen with a high incidence and mortality rate in both swine and humans. Following antibiotic treatment, the organism has evolved many resistance mechanisms, among of which efflux pump overexpression can promote drug extrusion out of the cell. This study clarified...