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ProteomicsMembrane modulation of super-secreting “midiBacillus” expressing the major Staphylococcus aureus antigen – a mass-spectrometry based absolute quantification approach.
Bacillus subtilis is a Gram-positive bacterium considered as a “cell-factory” for industrial enzymes and biopharmaceuticals. Given the commercial advantage of this organism, researchers have been working towards the improvement of this organism as a producer of secreted proteins. Nonetheless, there ...
ORGANISM(S): Bacillus subtilis subsp. subtilis 
2020-03-20 | PXD015496 | Pride
Srm Membrane proteins Heterologous protein production Proteomics Absolute quantification 
ProteomicsAriadne’s Thread in the Analytical Labyrinth of Membrane Proteins – Integration of Targeted and Shotgun Proteomics for Global Absolute Quantification of Membrane Proteins
The field of systems biology has been rapidly increasing in the past decade. However, the data produced by “omics” approaches is lagging behind the requirements of this field, especially when it comes to absolute abundances of membrane proteins. In the present study, a novel approach for large-scale...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2019-08-26 | PXD014272 | Pride
Shotgun-ms Absolute quantification Targeted-ms Snap-tag Bacillus subtilis Membrane proteins 
ProteomicsCell envelope adaptation and minimal Tat translocation are functionally intertwined
Protein secretion is key for bacterial growth, survival and adaptation. In the Gram-positive bacterium Bacillus subtilis, the twin-arginine (Tat) pathway serves in the exclusive secretion of proteins with bound cofactors. The Bacillus Tat pathway stands out for its minimalist nature with two indepen...
ORGANISM(S): Bacillus subtilis subsp. subtilis 
2022-08-11 | PXD012842 | Pride
Bacillus Liah Tatcy Twin-arginine Tatay Translocation Liars 
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