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TranscriptomicsChromatin immunoprecipitation of wild type yeast using anti-H3C and anti-pol II antibodies
Chromatin immunoprecipitation were performed in yeast WT cells using anti-H3C and anti-pol II antibodies
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-12 | E-MEXP-1951 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of mouse hepatocytes with anti-Hnf4alpha and anti-IgG antibodies
Chromatin from mouse hepatocytes was immunoprecipitated with antibodies against Hepatocyte Nuclear Factor 4 alpha (Hnf4a) and IgG. Amplified and labelled ChIP DNA and input DNA were hybridyzed to Mouse PromoterChip 5A.1 arrays.
ORGANISM(S): Mus musculus 
2009-04-01 | E-MEXP-1730 | biostudies-arrayexpress
ProteomicsNovel autoantibodies in rheumatoid arthritis using immunoprecipitation coupled with mass spectrometric analysis.
Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic joint inflammation resulting in progressive cartilage and bone destruction. In two thirds of early RA patients, rheumatoid factor (RF) and/or anti-citrullinated protein antibodies (ACPAs) are present. While these ant...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD069628 | Pride
Anti nuclear antibodies Mass spectrometry Ipms Rheumatoid arthritis Autoantibodies 
ProteomicsIn-depth data independent acquisition-mass spectrometry proteomic analysis of juvenile dermatomyositis serum samples reveals protein expression changes associated with muscle-specific autoantibodies
We analyzed sera from five JDM patients, each with three MSAs (anti-melanoma differentiation-associated protein 5 antibodies; anti-MDA5, anti-nuclear matrix protein 2 antibodies; anti-NXP2, or anti-transcriptional intermediary factor 1 alpha and/or gamma subunits antibodies; anti-TIF-1γ) and five he...
ORGANISM(S): Homo Sapiens (human) 
2023-04-23 | PXD036960 | JPOST Repository
TranscriptomicsChIP-chip of siGFP- or siHOTAIR-treated foreskin fibroblasts with anti-H3K4me2, anti-LSD1 or anti-SUZ12 antibodies on HOX tiling array
Long intergenic noncoding RNAs (lincRNAs) regulate chromatin states and epigenetic inheritance. Here we show that the lincRNA HOTAIR serves as a scaffold for at least two distinct histone modification complexes. A 5’ domain of HOTAIR binds Polycomb Repressive Complex 2 (PRC2) while a 3’ domain of H...
ORGANISM(S): Homo sapiens 
2010-07-08 | E-GEOD-22343 | biostudies-arrayexpress
GenomicsImpact of nuclear Piwi elimination on chromatin state in Drosophila melanogaster ovaries
The piRNA-interacting Piwi protein is involved in transcriptional silencing of transposable elements in ovaries of D. melanogaster. Here we characterized the genome-wide effect of nuclear Piwi elimination on the presence of the heterochromatic H3K9me3 mark and HP1a, as well as on the transcription-a...
ORGANISM(S): Drosophila melanogaster 
2014-05-19 | E-GEOD-56347 | biostudies-arrayexpress
TranscriptomicsChIP-chip of siGFP- or siHOTAIR-treated foreskin fibroblasts with anti-LSD1 or anti-SUZ12 antibodies on human HG18 Nimblegen promoter arrays
Long intergenic noncoding RNAs (lincRNAs) regulate chromatin states and epigenetic inheritance. Here we show that the lincRNA HOTAIR serves as a scaffold for at least two distinct histone modification complexes. A 5’ domain of HOTAIR binds Polycomb Repressive Complex 2 (PRC2) while a 3’ domain of H...
ORGANISM(S): Homo sapiens 
2010-07-07 | E-GEOD-22344 | biostudies-arrayexpress
ProteomicsProteome analysis of Co-IP samples from NCI-H1299 cell nuclear lysate with anti-AGO2 and anti-TNRC6A antibody.
Proteome analysis of Co-IP samples from NCI-H1299 cell nuclear lysate with anti-AGO2 and anti-TNRC6A antibody.
ORGANISM(S): Homo Sapiens (human) 
2021-02-06 | PXD017408 | JPOST Repository
TranscriptomicsChromatin immunoprecipitation of Arabidopsis 4 day-old light-treated seedlings using anti H3, CT, pan, anti H3K9me2 and IgG antibodies
ChIP was performed using 100 mg of 4 day-old light-treated seedlings. The following antibodies were used: anti H3, CT, pan (Millipore 07-690), anti H3K9me2 (Diagenode pAb-060-050) and IgG (Sigma I50006).
ORGANISM(S): Arabidopsis thaliana 
2009-12-08 | E-MEXP-2480 | biostudies-arrayexpress
TranscriptomicsExpression data of alloantigen stimulated splenocytes treated with leukocyte costimulatory blockade antibodies or no treatment
To elucidate the gene expression “footprint” of antigenically challenged T-cells which had been treated with anti-LFA-1, CTLA4Ig, anti-CD40-ligand antibodies, we performed microarray gene expression analysis comparing the expression profile of costimulatory blockade treated and untreated responder T...
ORGANISM(S): Mus musculus 
2011-02-03 | E-GEOD-26669 | biostudies-arrayexpress
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