Sort   by:  
 Page size 
OtherAssessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
Data from ProteomeXchange, PXD ID: PXD002534. File: GR_130129_20_OT3_NT_L3MBTL2_FAB_31_G11_2_HEK293.mzXML. Published as part of Nat Methods. 2015 Jun 29 . From the Abstract: {{i}} ... We describe a mass spectrometry-based standard operating procedure for scoring immunoprecipitation antibody quality...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320016573 | GPMDB
Resource Reanalysis 
ProteomicsA method to characterize antibody selectivity immunoprecipitation
Antibodies are used in multiple cell biology applications but there are methods to assess antibody quality, riskingdata integrity and repr developed a standard operating procedure using HEK293 cells to score and antibodies for suitability in immunoprecipitation. This method, validated in in fourind...
ORGANISM(S): Homo sapiens (Human) 
2015-07-15 | PXD002412 | Pride
Mass spectrometry Immunoprecipitation Antibody selectivity and specificity 
ProteomicsA method to characterize antibody selectivity immunoprecipitation, part 2
Antibodies are used in multiple cell biology applications but there are methods to assess antibody quality, riskingdata integrity and repr developed a standard operating procedure using HEK293 cells to score and antibodies for suitability in immunoprecipitation. This method, validated in in fourind...
ORGANISM(S): Homo sapiens (Human) 
2015-07-15 | PXD002534 | Pride
Mass spectrometry Immunoprecipitation Antibody selectivity and specificity 
GenomicsRNA Immunoprecipitation-sequencing from mouse liver using HuR antibody
RNA Immunoprecipitation-sequencing from mouse liver using HuR antibody
PRJNA601341 | ENA
TranscriptomicsHighly integrated epigenome maps in Arabidopsis - methylcytosine immunoprecipitation
Part of a set of highly integrated epigenome maps for Arabidopsis thaliana. Keywords: methylcytosine ChIP-chip on tiling array Identification of cytosine DNA methylation by methylcytosine immunoprecipitation and whole-genome oligonucleotide tiling microarrays for wildtype Columbia-0.
ORGANISM(S): Arabidopsis thaliana 
2008-04-20 | E-GEOD-10969 | biostudies-arrayexpress
ProteomicsA dataset describing a suite of novel antibody reagents for the RAS signaling network
RAS genes are frequently mutated in cancer and have for decades eluded effective therapeutic attack. The National Cancer Institute’s RAS Initiative has a focus on understanding pathways and discovering therapies for RAS-driven cancers. Part of these efforts is the generation of novel reagents to ena...
ORGANISM(S): Homo Sapiens 
2019-09-03 | PXD012604 | panorama
ProteomicsCav1_WT_KO immunoprecipitation MS
Immunoprecipitation was performed on Cav1 WT and KO cell line using Cav1 antibody. Proteins that interact with Cav1 were detected. Interleukin receptor (IR) antibody was also used on Cav1 WT and KO cells for immunoprecipitation. Proteins that interact with IR were detected.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-06-30 | MSV000089780 | MassIVE
TranscriptomicsChromatin immunoprecipitation of human LNCaP cells treated with synthetic androgen R1881 or ethanol (vehicle) using androgen receptor antibody
LNCaP cells were grown in charcoal stripped media for 48hrs and then treated for 1hr with the synthetic androgen R1881 or ethanol (vehicle). Cell extracts from both treatment conditions were then subjected to chromatin-immunoprecipitation using an anti-androgen receptor antibody, before labeling and...
ORGANISM(S): Homo sapiens 
2007-08-17 | E-TABM-233 | biostudies-arrayexpress
ProteomicsMass spectrometry (MS) datasets of QKI5 co-immunoprecipitation samples in THP-1 cells
We performed QKI5 co-immunoprecipitation followed by MS analysis to identify QKI5-interacting proteins in THP-1 cells.
ORGANISM(S): Homo sapiens (Human) 
2021-09-27 | PXD028743 | Pride
Qki5 co-immunoprecipitation  mass spectrometry  thp-1 cells 
ProteomicsA commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component
Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-...
ORGANISM(S): Mus musculus (Mouse) 
2020-12-14 | PXD022664 | Pride
Swi/snf Condensin Antibody Ncaph2 Cross-react 
Sort   by:  
 Page size