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GenomicsFlexible chromatin loops in the VH gene region of the Igh locus facilitate the generation of a diverse antibody repertoire
The immunoglobulin heavy-chain (Igh) locus undergoes large-scale contraction in pro-B cells, which facilitates VH-DJH recombination by juxtaposing distal VH genes next to the DJH- rearranged gene segment in the proximal Igh domain. By high-resolution mapping of long-range interactions, we now demons...
ORGANISM(S): Mus musculus 
2013-09-30 | E-GEOD-43008 | biostudies-arrayexpress
TranscriptomicsA Highly Sensitive and Unbiased Approach for Elucidating Antibody Repertoires
Developing B lymphocytes undergo V(D)J recombination to assemble germline V, D, and J gene segments into exons that encode the antigen-binding variable region of immunoglobulin (Ig) heavy (H) and light (L) chains. IgH and IgL chains associate to form the B cell receptor (BCR), which upon antigen bi...
ORGANISM(S): Mus musculus 
2016-07-22 | E-GEOD-82126 | biostudies-arrayexpress
GenomicsGenetic measurement of memory B-cell recall using antibody repertoire sequencing

We developed an improved high throughput sequencing approach to measure the quantities and sequences of the repertoire of antibody heavy chain RNA in a blood sample. Using this approach we analyzed the antibody repertoire in response to yearly vaccinations with influenza vaccines TIV and LAIV in ...

phs000656.v1.p1 | EGA
ProteomicsLongitudinal proteomic analysis of influenza HA-reactive IgG antibody repertoire over multiple years and repeated vaccinations
Longitudinal proteomic analysis of H1N1 A/California/7/2009 (CA09) hemagglutinin (HA)-reactive serum IgG antibody repertoire over 5 years and multiple (yearly) vaccinations (both pre- and post-vaccination samples) in a donor. Dataset consists of steady-state (pre-vaccination at day 0) and peak-respo...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-11-08 | MSV000083120 | MassIVE
TranscriptomicsAntibody mRNA-seq repertoire sequencing to study relapses after B-cell depletion therapy
Rituximab (RTX) is widely used as a first-line therapeutic strategy for patients affected by immune thrombocytopenia (ITP). However, a large proportion of patients relapse after successful treatment. The present NGS assay was done to help find the cause for this relapse on the immune repertoire leve...
ORGANISM(S): Homo sapiens 
2021-01-18 | E-MTAB-9953 | biostudies-arrayexpress
TranscriptomicsSingle-cell immune repertoire sequencing of bone marrow plasma cells following infection and immunizations
We performed single-cell transcriptome and antibody repertoire sequencing of bone marrow plasma cells following protein (OVA and TNFR2) immunizations or infection with high dose LCMV clone 13.
ORGANISM(S): Mus musculus 
2021-12-01 | E-MTAB-11096 | biostudies-arrayexpress
ProteomicsNext-generation Serology by Mass Spectrometry:Readout of the SARS-CoV-2 Antibody Repertoire
Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses new advances i...
ORGANISM(S): Homo Sapiens 
2022-09-24 | PXD028627 | panorama
TranscriptomicsAntibody repertoire RNA-seq throughout key stages of mouse B-cell development (pre-B-cells, naive B cells, plasma cells)
We sought to investigate the extent of genetic and antigen-driven predetermination of antibody repertoires throughout B-cell development (pre-B cells, naive B cells, plasma cells). To this end, we used (i) an inbred model organism (mouse, C57BL/6J) to quantify genetic predetermination and (ii) cohor...
ORGANISM(S): Mus musculus 
2017-03-28 | E-MTAB-5349 | biostudies-arrayexpress
ProteomicsBenchmarking of mass spectrometry-based antibody repertoire profiling reveals extensive systematic biases
The serum antibody repertoire is crucial for long-term immune protection, holding significant immunological and biotechnological value. Bottom-up proteomics is the most widely used mass spectrometry (MS) technique for profiling the sequence diversity of serum antibodies. However, serum antibody bott...
ORGANISM(S): Homo sapiens (Human) 
2025-12-04 | PXD055846 | Pride
Serum antibody bottom-up proteomics (ab-seq) Ms Antibody sequencing Serum antibody repertoire 
GenomicGenetic Measurement of Memory B-cell Recall Using Antibody Repertoire Sequencing

We developed an improved high throughput sequencing approach to measure the quantities and sequences of the repertoire of antibody heavy chain RNA in a blood sample. Using this approach we analyzed the antibody repertoire in response to yearly vaccinations with influenza vaccines TIV and LAIV in ...

phs000656 | dbGaP
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