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ProteomicsPATCH labeling in the BSA through red light
For the liquid chromatography-mass spectrometry (LC-MS) analysis, the 500 μM AP was used to label BSA in the reaction, followed by clicking chemical conjugation with 0.1 mM azide-PEG3-biotin under 0.5 mM CuCl(2), 2 Mm Tris (3-hydroxypropyltriazolylmethyl) amine (THPTA, Sigma-Aldrich) and 5 mM ascorb...
ORGANISM(S): Bos taurus (Bovine) 
2025-07-30 | PXD057016 | Pride
Pcn bsa 
ProteomicsTemporal Proteomic and Lipidomic Profiles of Cerulein-Induced Acute Pancreatitis Reveals Novel Insights for Metabolic Alterations in the Disease Pathogenesis
The pathophysiological mechanisms of acute pancreatitis (AP) are complex and have remained a mystery to date, but metabolism is gradually recognized as an important driver of AP onset and development. We used a cerulein-induced AP mouse model to conduct LC-MS/MS based time-course proteomics and lipi...
ORGANISM(S): Mus Musculus 
2022-12-06 | PXD038567 |
UnknownTandem mass spectrometry-based detection of c4'-oxidized abasic sites at specific positions in DNA fragments.
Not available
S-EPMC3838791 | biostudies-literature
ProteomicsMapping ISG15 sites on GAPDH and PGK1 by AP-MS
We employed an AP-MS approach to map ISG15 modification sites on GAPDH and PGK1 in HEK293T cells by overexpression of FLAG-GAPDH, FLAG-PGK1 or FLAG-GFP together with the ISG15 conjugation enzymes (E1, E2, E3) and wild-type HA-ISG15 or conjugation-defective ISG15-AA. Cells were lysed in physiological...
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD071724 | Pride
Ap-ms Pgk1 Gapdh Isg15 Glycolysis 
UnknownIn vitro replication and thermodynamic studies of methylation and oxidation modifications of 6-thioguanine.
Not available
S-EPMC1920245 | biostudies-literature
UnknownUncertainty Evaluation for the Quantification of Urinary Amphetamine and 4-Hydroxyamphetamine Using Liquid Chromatography-Tandem Mass Spectrometry: Comparison of the Guide to the Expression of Uncertainty in Measurement Approach and the Monte Carlo Method with R.
Not available
S-EPMC10574584 | biostudies-literature
UnknownTAZ interactome analysis using nanotrap-based affinity purification-mass spectrometry.
Not available
S-EPMC11928053 | biostudies-literature
UnknownPotential biomarker proteins for aspiration pneumonia detected by shotgun proteomics using buccal mucosa samples: a cross-sectional case-control study.
Not available
S-EPMC9996945 | biostudies-literature
UnknowniPhos: a toolkit to streamline the alkaline phosphatase-assisted comprehensive LC-MS phosphoproteome investigation.
Not available
S-EPMC4290636 | biostudies-literature
UnknownplncRNA-encoded pep-AP attenuates the pentose phosphate pathway and sensitizes colorectal cancer cells to Oxaliplatin
Not available
S-SCDT-EMBOR-2021-53140-T | biostudies-other
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