Apoplexy - OmicsDI

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Single nuclei were captured using the 10x Genomics Chromium Controller. (3)
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- (3)
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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (1)
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CHEBI ID

CHEBI:17754 (4)
CHEBI:17126 (3)
CHEBI:17234 (3)
CHEBI:28044 (3)
CHEBI:16958 (3)
CHEBI:32816 (3)
CHEBI:78320 (3)
CHEBI:15428 (3)
CHEBI:16919 (3)
CHEBI:15354 (3)
CHEBI:30769 (3)
CHEBI:20067 (3)
CHEBI:48430 (2)
CHEBI:17822 (2)
CHEBI:26271 (2)
CHEBI:15356 (2)
CHEBI:22660 (2)
CHEBI:27570 (2)
CHEBI:30751 (2)
CHEBI:18186 (2)
CHEBI:26986 (2)
CHEBI:28300 (2)
CHEBI:24898 (2)
CHEBI:25017 (2)
CHEBI:1148 (2)
CHEBI:27266 (2)
CHEBI:29016 (2)
CHEBI:16704 (2)
CHEBI:15366 (2)
CHEBI:72998 (2)

Metabolite Name

Taurine (6)
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Creatinine (3)
L-Tyrosine (3)
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Triterpenoid (2)
Cholesterol sulfate (2)
LysoPC(P-18:0/0:0) (2)
LysoPC(O-18:0/0:0) (2)

First Public Date

2020 (3)
2022 (2)
2018 (1)
2014 (1)

Modification

15.994915 (2)
42.010565 (2)
57.021464 (2)

Study type

Transcription profiling by array (144)
ChIP-seq (35)
RNA-seq of coding RNA (33)
ChIP-chip by tiling array (25)
RNA-seq of coding RNA from single cells (22)
Other (10)
Single nucleus RNA sequencing (8)
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ChIP-chip by array (6)
ATAC-seq (6)
DNA-seq (5)
Unknown experiment type (4)
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ScATAC-seq (3)
Animal - One-color microarray (2)
Animal - High-throughput sequencing (2)
Methylation profiling by array (2)
Methylation profiling by high throughput sequencing (2)
Single-nucleus ATAC-seq (1)
MeDIP-seq (1)
One-color microarray (1)
Cell line - One-color microarray (1)
High-throughput sequencing (1)
Transcription profiling by RT-PCR (1)
Ribo-seq (1)

Release Date

2013 (47)
2010 (40)
2014 (31)
2015 (30)
2011 (23)
2023 (22)
2008 (22)
2016 (19)
2012 (19)
2025 (17)
2026 (12)
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2022 (10)
2020 (9)
2007 (9)
2009 (8)
2019 (7)
2018 (6)
2006 (4)
2017 (2)
2005 (1)
2004 (1)

Lab affiliation

Université de Strasbourg; Institut Pasteur de Lille (P3M) (2)

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Plant diseases "Esca proper and apoplexy"

Physiological changes in green stems of Vitis vinifera L. cv.Chardonnay in response to esca proper and apoplexy revealed by proteomic and transcriptomic analyses

ORGANISM(S): Viridiplantae

2012-02-01 | PRD000582 | Pride

Esca proper and apoplexy on trunk wood of Vitis vinifera L. cv.Chardonnay

Physiological changes in trunk wood of Vitis vinifera L. cv. Chardonnay in response to esca proper and apoplexy revealed by proteomic and transcriptomic analyses

ORGANISM(S): Vitis

2014-06-25 | PXD000015 | Pride