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AraC is an Escherichia coli transcription factor that regulates genes in response to the presence/absence of arabinose. We used transcription profiling to determine RNA levels in Escherichia coli K-12 strain MG1655 and MG1655 delta araC grown either in the absence or the presence of arabinose. Thus,...
ORGANISM(S): Escherichia coli K-12 
We used RNA-seq to determine the effects of AraC and arabinose on RNA levels genome-wide in S. enterica. Wild-type or delta araC mutant cells were grown in both the presence and absence of 0.2% L-arabinose. RNA-Seq libraries from two independent biological replicate samples were sequenced for (i) wi...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. 14028s Wu 
We mapped the genome-wide binding of C-terminally FLAG-tagged AraC in S. enterica subsp. enterica serovar Typhimurium strain 14028s using ChIP coupled with deep sequencing (ChIP-seq). We identified five putative target loci for AraC: upstream of araB/araC, araE, araJ, STM14_0178, and within sseD.
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S 
A new approach to sample preparation and enzymatic digestion in bottom-up proteomics has been developed using alginate-based hydrogel entrapment of enzymes. This hydrogel facilitates rapid and room-temperature digestions with multienzyme capabilities. Three methodologies were tested: within microcen...
ORGANISM(S): Homo sapiens (Human) 
2024-11-12 | PXD052369 | Pride
Properly integrating multiple modalities allows for a deeper understanding of complex biological processes. This study presents an unsupervised method of integrating proteomics, lipidomics, and metabolomics to reveal cross-omic trends, often overlooked by traditional analysis methods, in pituitary p...
ORGANISM(S): Equus caballus (Horse) 
2026-08-13 | PXD062230 | Pride
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