To figure out the major lysine residues of PD-L1 ubiquitinated by ARIH1, mass spectrometry was utilized to identify the ubiquitination site. K271 was identified as the major residues of ubiquitination
To explore the molecular mechanisms how ARIH1 activates CD8+ T cells and enhances PD-L1 blockade therapy, we performed RNA sequencing (RNA-seq) to determine whether Arih1 overexpression affects the gene expression in 4T1 cells.
