Sort   by:  
 Page size 
ProteomicsRapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons
Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells. Here, we develop CRISPR-engineered mouse lines that enable rapid and highly specific degradation of tagged endogenous proteins in vivo. Most...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-07 | PXD032374 | Pride
Dia Condensin Auxin degradation 
MetabolomicsUntargeted lipidomics analysis of yeast metabolic proteins degradations to investigate the role of metabolic proteins in lipid metabolism
We combine auxin-inducible degradation of 500 characterized and 204 uncharacterized proteins with untargeted lipidomics providing a quantitative readout of cellular lipidomic states.
2026-03-24 | MTBLS14098 | MetaboLights
ProteomicsInducible degradation-coupled phosphoproteomics identifies PP2A-Rts1 as a novel eisosome regulator
We developed and validated a workflow in S. cerevisiae combining auxin-inducible protein degradation with LC-MS/MS phosphoproteomics and used it to identify candidate phosphatase substrates during mitosis, when kinase activity is high. Our results revealed that PP2A-Rts1 influences dephosphorylation...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-10-24 | PXD044337 | Pride
Phosphoproteomics Pp2a Rts1 Phosphatase Auxin inducible degradation 
ProteomicsDecoding novel acquired resistance mechanisms in auxin-inducible protein degradation
Loss of function studies played a critical role in dissecting gene function and downstream regulation networks. However, the most used knockout approaches, including CRISPR/Cas9, Cre/LoxP, shRNAs, and more, require days to achieve protein depletion. This will lead to the concern of the secondary eff...
ORGANISM(S): Homo sapiens (Human) 
2026-08-24 | PXD060834 | Pride
Lc-msms 
GenomicsDeciphering resistance mechanisms to auxin-inducible protein degradation in mammalian cells [ChIP-Seq]
Deciphering resistance mechanisms to auxin-inducible protein degradation in mammalian cells [ChIP-Seq]
PRJNA1314713 | ENA
GenomicsDeciphering resistance mechanisms to auxin-inducible protein degradation in mammalian cells [RNA-Seq]
Deciphering resistance mechanisms to auxin-inducible protein degradation in mammalian cells [RNA-Seq]
PRJNA1226232 | ENA
ProteomicsPhosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55
Protein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. We here employed auxin-inducible degradation of S. cerevisiae PP2A regulatory subunit Cdc55 in co...
ORGANISM(S): Saccharomyces Cerevisiae 
2021-01-27 | PXD019534 | panorama
TranscriptomicsTranscriptome profiling of A. thaliana expressing auxin degradation resistant IAA26-P108H-GFP from the IAA26 native promoter
The goal of this study was to compare the transcriptional profile (RNA-seq) of seven-week-old auxin resistant pIAA26::IAA26-P108H-GFP transgenic plants to non-transformed control plants to identify candidate genes and pathways regulated by IAA26. RNA-seq analysis was performed with A. thaliana ecoty...
ORGANISM(S): Arabidopsis thaliana 
2016-04-14 | E-GEOD-75983 | biostudies-arrayexpress
UnknownRapid, efficient auxin-inducible protein degradation in Candida pathogens.
Not available
S-EPMC10245712 | biostudies-literature
GenomicsDeciphering resistance mechanisms to auxin-inducible protein degradation in mammalian cells [ChIP-Seq]
Targeted protein degradation is a favorable strategy for studying the immediate downstream effects of protein loss-of-function, such as the auxin-inducible degron (AID) system. Although this system has been applied extensively to cell and animal models, degradation resistance to long-term auxin trea...
ORGANISM(S): Homo sapiens 
2026-07-08 | GSE307186 | GEO
Sort   by:  
 Page size