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ProteomicsVariation in auxin sensing guides AUX/IAA repressor ubiquitylation and destruction
Auxin is a drug-like small molecule and morphogen that triggers the formation of SCFTIR1/AFB-AUX/IAA co-receptor complexes leading to ubiquitylation and proteasome-dependent degradation of AUX/IAA transcriptional repressors in plants. Here, we systematically dissect auxin sensing by SCFTIR1-IAA6 and...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-06-14 | PXD004027 | Pride
Phytohormone Ubiquitylation Auxin Scf-type e3 ligase Ubiquitination Tir1/afbs Proteolysis Aux/iaa 
ProteomicsEnsured auxin sensing by degron flexibility
Using a structural proteomics approach via chemical cross-linking combined with mass spectrometry (XL-MS), we have resolved SCFTIR auxin·AUX/IAA complex overall topology, and established the power of IDRs modulating auxin receptor assemblies.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-05-11 | PXD015285 | Pride
Auxin Cross-linking Iaa12 Auxin receptor Xl-ms Idrs Indole-3-acetic acid Aux/iaas Iaa7 Scf-type e3 ubiquitin ligase Tir1 
TranscriptomicsTranscription profiling of Arabidopsis seedlings treated with glucose and indole-3-acetic acid (auxin) to study glucose-auxin interactions
To find out the extent of interaction between glucose and auxin signaling
ORGANISM(S): Arabidopsis thaliana 
2009-07-10 | E-MEXP-1703 | biostudies-arrayexpress
ProteomicsABLs and TMKs are co-receptors for extracellular auxin
Extracellular perception of auxin, an essential phytohormone in plants, has been debated for decades. Auxin binding protein 1 (ABP1) physically interacts with quintessential transmembrane kinases (TMKs) and was proposed to act as an extracellular auxin receptor, but its role was disputed because abp...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-11-25 | PXD044623 | Pride
Auxin receptor Arabidopsis 
ProteomicsABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization
The phytohormone auxin triggers transcriptional reprograming utilizing a well-characterized nuclear perception machinery. In contrast, mechanisms underlying other auxin effects, such as auxin feed-back on its transport, rapid regulation of ion fluxes or ultrafast global phospho-response, remain enig...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-08-25 | PXD031063 | Pride
Auxin abp1 tmk1 phosphoproteomics 
TranscriptomicsTranscription profiling of Arabidopsis seedlings treated with auxin
Five-day old light-grown Arabidopsis seedlings were immersed 10 µM indole-3-acetic acid (auxin) or water
ORGANISM(S): Arabidopsis thaliana 
2008-06-16 | E-GEOD-863 | biostudies-arrayexpress
TranscriptomicsABP1-regulated gene expression in etiolated Arabidopsis seedlings
The AUXIN BINDING PROTEIN 1 (ABP1) is an essential plant protein involved in the control of growth and development all along the plant life. This protein was initially identified on its capacity to bind the phytohormone auxin. Binding of auxin to ABP1 was shown to enhance cell expansion of leaf cell...
ORGANISM(S): Arabidopsis thaliana 
2015-04-07 | E-GEOD-46196 | biostudies-arrayexpress
ProteomicsDegron flexibility enforces auxin sensing
Cullin RING-type E3 ubiquitin ligase SCFTIR1/AFB1-5 and their ubiquitylation targets, AUX/IAAs, sense auxin concentrations in the nucleus. TIR1 binds a surface- exposed degron in AUX/IAAs promoting their ubiquitylation and rapid auxin-regulated proteasomal degradation. Here, we resolved TIR1·auxin·I...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-05-27 | PXD015392 | Pride
Degron Auxin Phytohormones Aux/iaa Scftir1 Intrinsic disorder Allostery 
TranscriptomicsAuxin stimulates brassinosteroid biosynthesis in Arabidopsis roots
We found that auxin stimulates gene expression of DWF4, which encodes a rate-dertermining step in brassinosteroid biosynthesis pathways. This increased gene expressioin subsequently led to elevation of the biosynthetic flux in Arabidopsis roots. To determine the list of genes that are regulated by a...
ORGANISM(S): Arabidopsis thaliana 
2011-02-15 | E-GEOD-12964 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis wild type and ein2-5 seedlings to investigate ethylene and auxin interactions in the root
Understanding how developmental and environmental signals are integrated to produce specific responses is one of the main challenges of modern biology. Hormones and, most importantly, interactions between different hormones serve as crucial regulators of plant growth and development, playing central...
ORGANISM(S): Arabidopsis thaliana 
2008-06-15 | E-GEOD-7432 | biostudies-arrayexpress
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