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ProteomicsRBM6 proximity interactions (RBM6-APEX)
We sought to map RBM6 interactome using ascorbate peroxidase (APEX2)-based proximity labelling combined with mass spectrometry. Toward this end, we used CRISPR-cas9 methodology to establish MCF10A cell line expressing APEX2 fused to the N-terminal of RBM6, hereafter called MCF10AAPEX2-RBM6. The pero...
ORGANISM(S): Homo sapiens (Human) 
2021-12-29 | PXD024295 | Pride
Rbm6   apex2   proximity interactions 
ProteomicsC8orf33 dictates DNA double-strand break repair choice by modulating KAT8-mediated H4K16 acetylation
DNA double-strand breaks (DSBs) are repaired through distinct pathways, including homologous recombination (HR) and non-homologous end joining (NHEJ). The regulation of repair pathway choice is crucial for maintaining genomic stability and preventing carcinogenesis. Consequently, there is growing in...
ORGANISM(S): Homo sapiens (Human) 
2025-10-29 | PXD062780 | Pride
Acetylation Dna double-strand break 
ProteomicsCharacterization of RBM10 interactome in unchallenged and MK1775-treated cells
Mapping of RBM10-interactome using GFP-trap purification of EGFP-RBM10 expressed in HEK293T cells before and after WEE1 inhibition by MK1775. HEK293T expressing EGFP only or EGFP-RBM10 fusion (2 main isoforms) were left untreated or treated with 300nM MK1775 for 4 hours prior to GFP-trap enrichment ...
ORGANISM(S): Homo sapiens (Human) 
2024-07-16 | PXD040109 | Pride
Rbm10 wee1 mk1775 luad 
GenomicsChIP-seq mapping of KAT8 acetylatransferase at AsiSI-induced DNA double strand break sites
C8orf33-proficient and deficient DIvA cells were treated with 4-hydroxy tamoxifen (4OHT) to induce DNA double strand breaks (DSB) at several loci within the human genome. following 4OHT treatment cells were subject to ChIP-seq analysis for KAT8 acetyltransferase to map its enrichment at DSB sites in...
ORGANISM(S): Homo sapiens 
2025-04-10 | E-MTAB-15055 | biostudies-arrayexpress
GenomicsChIP-seq mapping of gH2AX, PanKcr, H3K9cr, and ENL at AsiSI-induced double-strand breaks in control and CDYL1-depleted U2OS-DIvA cells
Control and CDYL1-depleted U2OS-DIvA cells were treated with 4-hydroxy tamoxifen (4OHT) to induce multiple DNA double-strand breaks (DSBs) at defined loci in the genome via AsiSI restriction enzyme. This system was used to map the changes in lysine crotonylation (Kcr) and ENL at DSB sites in control...
ORGANISM(S): Homo sapiens 
2022-01-22 | E-MTAB-10550 | biostudies-arrayexpress
GenomicsGenome-wide CRISPR-Cas9 screen in isogenic parental and RBM10-KO HCC827 cells
Genome-wide CRISPR-Cas9 knockout screen using TKOv1 sgRNA library performed in isogenic RBM10-proficient and RBM10-deficient HCC827 cells.
ORGANISM(S): Homo sapiens 
2024-03-01 | E-MTAB-13626 | biostudies-arrayexpress
GenomicsCRISPR-Cas9 RBM10 synthetic lethality screen
Genome-wide CRISPR-Cas9 knockout screen using TKOv1 sgRNA library was performed in isogenic RBM10-proficient and RBM10-deficient HCC827 cells.
ORGANISM(S): Homo sapiens 
2023-03-04 | E-MTAB-12724 | biostudies-arrayexpress
TranscriptomicsRNA-seq of RBM10 in LUAD cell lines
RNA-seq in isogenic RBM10-proficient and RBM10-deficient cells derived from lung adenocarcinoma cell lines HCC827 (parental and RBM10 knockout; control siRNA and RBM10 siRNA) and NCI-H1299 (parental and RBM10 knockout).
ORGANISM(S): Homo sapiens 
2024-04-01 | E-MTAB-13617 | biostudies-arrayexpress
TranscriptomicsRNA-seq of RBM42-proficient and -deficient HCT116 cell line after VP16 treatment
To explore the role of RBM42 in the DNA damage response, we sought to determine the transcriptome of RBM42-proficient and -deficient cells following DNA damage induction. RNA samples were prepared from etoposide (VP16) treated HCT116 cells transfected with control or RBM42 siRNA, and subjected RNA-s...
ORGANISM(S): Homo sapiens 
2022-12-15 | E-MTAB-11877 | biostudies-arrayexpress
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