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ProteomicsDifferences in proteasome targeting in Arabidopsis leaf mesophyll, epidermal and vascular cells
For the detailed and sensitive detection of the responses to a specific treatment it is important to perform tissue or cell type specific analyses, but this is not easily achievable for the different leaf tissues. Here we developed a method termed MeSelect to effectively separate leaf epidermis, vas...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-05-13 | PXD000942 | Pride
Ubiquitylation Arabidopsis Syringolin a Affinity enrichment Vasculature Mesophyll Ubiquitin proteasome system Leaf tissues Quantitative proteomics Epidermis 
ProteomicsDifferences in proteasome targeting in Arabidopsis leaf mesophyll, epidermal and vascular cells
For the detailed and sensitive detection of the responses to a specific treatment it is important to perform tissue or cell type specific analyses, but this is not easily achievable for the different leaf tissues. Here we developed a method termed MeSelect to effectively separate leaf epidermis, vas...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-05-14 | PXD000941 | Pride
Ubiquitylation Arabidopsis Syringolin a Affinity enrichment Vasculature Mesophyll Ubiquitin proteasome system Leaf tissues Quantitative proteomics Epidermis 
ProteomicsProtein abundance changes and ubiquitylation targets identified upon inhibition of the proteasome with Syringolin A
The numerous roles of the ubiquitin proteasome system (UPS) in cellular control have triggered interest in the identification of these ubiquitylation targets and their sites of ubiquitylation. For the identification of ubiquitylated proteins we employed affinity enrichment using an ubiquitin binding...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2014-04-15 | PXD000565 | Pride
Ubiquitylation Syringolin a Affinity enrichment Ubiquitin proteasome system Quantitative proteomics 
ProteomicsProtein abundance changes and ubiquitylation targets identified upon inhibition of the proteasome with Syringolin A
The numerous roles of the ubiquitin proteasome system (UPS) in cellular control have triggered interest in the identification of these ubiquitylation targets and their sites of ubiquitylation. For the identification of ubiquitylated proteins we employed affinity enrichment using an ubiquitin binding...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2014-04-15 | PXD000566 | Pride
Ubiquitylation Syringolin a Affinity enrichment Ubiquitin proteasome system Quantitative proteomics 
ProteomicsProtein abundance changes and ubiquitylation targets identified upon inhibition of the proteasome with Syringolin A
The numerous roles of the ubiquitin proteasome system (UPS) in cellular control have triggered interest in the identification of these ubiquitylation targets and their sites of ubiquitylation. For the identification of ubiquitylated proteins we employed affinity enrichment using an ubiquitin binding...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2014-05-19 | PXD000568 | Pride
Ubiquitylation Syringolin a Affinity enrichment Ubiquitin proteasome system Quantitative proteomics 
ProteomicsProtein abundance changes and ubiquitylation targets identified upon inhibition of the proteasome with Syringolin A
The numerous roles of the ubiquitin proteasome system (UPS) in cellular control have triggered interest in the identification of these ubiquitylation targets and their sites of ubiquitylation. For the identification of ubiquitylated proteins we employed affinity enrichment using an ubiquitin binding...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2014-04-15 | PXD000567 | Pride
Ubiquitylation Syringolin a Affinity enrichment Ubiquitin proteasome system Quantitative proteomics 
ProteomicsProtein abundance changes and ubiquitylation targets identified upon inhibition of the proteasome with Syringolin A
The numerous roles of the ubiquitin proteasome system (UPS) in cellular control have triggered interest in the identification of these ubiquitylation targets and their sites of ubiquitylation. For the identification of ubiquitylated proteins we employed affinity enrichment using an ubiquitin binding...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2014-04-15 | PXD000521 | Pride
Ubiquitylation Syringolin a Affinity enrichment Ubiquitin proteasome system Quantitative proteomics 
ProteomicsArabidopsis thaliana leaf wound proteomics
Not available
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2011-06-23 | PRD000280 | Pride
ProteomicsGFPtrap enrichment of CYP83A1 and CYP83B1
CYP83A1 and CYP83B1 are important enzymes in the biosynthesis of glucosinolates. However, only few of their interaction partners are known so far. We therefore performed a GFPtrap affinity enrichment using protein extracts from lines expressing tagged CYP83A1 or CYP83B1, or from wild type plants as ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-11-30 | PXD005585 | Pride
Arabidopsis Seedling Affinity enrichment Glucosinolate biosynthesis Cyp83b1 Cyp83a1 Gfptrap 
ProteomicsProtein accumulation in Arabidopsis APG mutants
Not available
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2012-10-03 | PRD000737 | Pride
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