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ProteomicsGFPtrap enrichment of CYP83A1 and CYP83B1
CYP83A1 and CYP83B1 are important enzymes in the biosynthesis of glucosinolates. However, only few of their interaction partners are known so far. We therefore performed a GFPtrap affinity enrichment using protein extracts from lines expressing tagged CYP83A1 or CYP83B1, or from wild type plants as ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-11-30 | PXD005585 | Pride
Arabidopsis Seedling Affinity enrichment Glucosinolate biosynthesis Cyp83b1 Cyp83a1 Gfptrap 
GenomicsTranscriptome-wide mapping of RNA:protein interactions of GFP-RS2Z35 and GFP-RS2Z36 in tomato (Solanum lycopersicum) leaves.
Transcriptome-wide mapping of RNA:protein interactions of GFP-RS2Z35 and GFP-RS2Z36 in tomato (Solanum lycopersicum) leaves.
PRJNA1082655 | ENA
ProteomicsAutophagosome Proteomics of HEK293 cells
Pan-caspase inhibitor Z-VAD-fmk acts as an inhibitor of peptide:N-glycanase (NGLY1); an endoglycosidase which cleaves N-linked glycans from glycoproteins exported from the endoplasmic reticulum during ER-associated degradation (ERAD). Pharmacological N-glycanase inhibition by Z-VAD-fmk or siRNA knoc...
ORGANISM(S): Homo sapiens (Human) 
2022-01-12 | PXD020367 | Pride
Autophagy  autophagosome  ngly1  n-glycanase  z-vad-fmk  off-target 
ProteomicsImmunoprecipitation-mass spectrometry to identify Gdown1-associating proteins in the cytoplasm and nucleoplasm
Before RNA polymerase II (Pol II) can enter the nucleus, where it transcribes messenger RNA, it must first be assembled in the cytoplasm. We have performed proteomic and structural analysis of endogenous human cytoplasmic Pol II complexes, enriched in known biogenesis factors including RPAP2 and the...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD071638 | Pride
Lc-msms K562 Human 
TranscriptomicsThe human nuclear exosome targeting complex is loaded onto newly synthesized RNA to direct early ribonucleolysis
Individual-nucleotide resolution UV-crosslinking and immunoprecipitation (iCLIP) followed by high-throughput sequencing of RBM7-associated transcripts. Note: these data relate to Figure 1, 2, 3, 4, 5 and 6 in Lubas, Andersen et al., Cell Reports 2014 RBM7-associated transcripts
ORGANISM(S): Homo sapiens 
2014-12-03 | E-GEOD-63791 | biostudies-arrayexpress
ProteomicsCo-immunoprecipitation and proteomic analysis of Major Histocompatibility Complex Class I-related (MR1) mutants
Identification of cellular proteins mediating presentation of bacterial antigens by the MHC I-related protein MR1. MR1 mutant proteins that differ in their capacity to present antigens from different sources were immunoprecipitated and their interacting proteins were compared to identify candidate p...
ORGANISM(S): Homo sapiens (Human) 
2026-04-06 | PXD062277 | Pride
Co-immunoprecipitation Antigen presentation Mr1 Endosomal trafficking 
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