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ProteomicsA structural model of the human mSWI/SNF (BAF) complex bound to the nucleosome informs disease mechanisms
Mammalian SWI/SNF complexes are multi-component ATP-dependent chromatin-remodeling complexes that regulate genomic architecture. Here we present the first structural model of the human canonical BAF chromatin-remodeling complex bound to a nucleosome generated using cryo-EM, cross-linking mass spectr...
ORGANISM(S): Homo sapiens (Human) 
2021-02-10 | PXD020992 | Pride
Homology model Cryo-em Atp-dependent chromatin remodeling Nucleosome acidic patch Yeast swi/snf Mammalian swi/snf Canonical baf complex Human disease Mutations 
ProteomicsAcute BAF perturbation causes immediate changes in chromatin accessibility
Loss-of-function mutations in genes coding for subunits of the large, multifarious BRG1/BRM associated factor (BAF) chromatin remodeling complexes are frequently causative for cancer or developmental diseases1-5. Cells lacking the most frequently mutated subunits like the ATPase SMARCA4 typically ex...
ORGANISM(S): Homo sapiens (Human) 
2020-12-23 | PXD018381 | Pride
Atac-seq Enhancer Dtag knock-in Chromatin remodeling Baf complex Chromatin accessibility Acute chemical degradation Synthetic lethality Mammalian swi/snf complex Kinetics 
ProteomicsSystematic characterization of BAF mutations explains intra-complex synthetic lethalities
Aberrations in genes coding for subunits of the BAF chromatin remodeling complex are highly abundant in human cancers. Currently, it is not understood how these loss-of-function mutations contribute to cancer development and how they can be targeted therapeutically. The cancer type specific occurren...
ORGANISM(S): Homo sapiens (Human) 
2019-08-01 | PXD013102 | Pride
Atac-seq Interaction proteomics Chromatin remodeling Baf complex Chromatin accessibility Chromatin binding Transcriptomics Synthetic lethality Mammalian swi/snf complex 
ProteomicsMudPIT analyses of the proteins co-purified with components of the LSD1 (KDM1A) transcriptional repressor complex and the non-canonical BAF complex, immuno-precipitated from MKL-1 and WaGa MCC cell lines
Protein Complexes Purification: Immunoprecipitations were performed with antibodies to LSD1 (2139; Cell signaling technology), INSM1 Mouse (SC-271408, Santa Cruz Biotechnology), RCOR2 (23969-1-AP; Proteintech Group), BRD9 (61537; Active Motif Rabbit), and normal Rabbit IgG (2729; Cell signaling tech...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-01-25 | MSV000083364 | MassIVE
ProteomicsA non-canonical BRD9-containing BAF chromatin remodeling complex regulates naïve pluripotency in mouse embryonic stem cells
The mammalian BRG1-associated factors (BAF) complex is a multi-subunit chromatin remodeling complex that is an important component of the embryonic stem cell (ESC) transcriptional regulatory network. However, the role of individual subunits in BAF complex targeting and function needs to be elucidate...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-18 | PXD010670 | Pride
Baf Chromatin remodeling 
GenomicsBAF250a regulates nucleosome occupancy and histone modification in priming embryonic stem cell differentiation
BAF complex is one major group of chromatin remodeling factors in mammals. However, how BAF regulated nucleosomes and other histone modifications is not clear. Here we delete BAF250a, a major component in esBAF to study the nucleosome and histone changes in ESCs. We find that deletion of BAF250a lea...
ORGANISM(S): Mus musculus 
2015-08-03 | E-GEOD-59082 | biostudies-arrayexpress
GenomicsSS18-SSX-mediated hijacking of BAF complexes drives synovial sarcoma
Synovial sarcoma (SS) is defined by a recurrent t(x;18) chromosomal translocation, which produces the hallmark SS18-SSX oncogenic fusion. Incorporation of SS18-SSX into BAF complexes renders BAF complexes aberrant in two distinct manners: the addition of 78aa of SSX onto SS18, and concomitant loss o...
EGAS00001002920 | EGA
GenomicsThe BAF complex blocks Myogenesis in rhabdomyosarcoma
The BAF complex blocks Myogenesis in rhabdomyosarcoma
PRJNA680368 | ENA
ProteomicsThe BAF complex blocks Myogenesis in rhabdomyosarcoma
Rhabdomyosarcoma (RMS) is a highly malignant pediatric cancer of skeletal muscle lineage. The aggressive alveolar subtype is commonly characterized by t(2;13) or t(1;13) translocations with the expression of PAX3- or PAX7-FOXO1 fusion transcription factors respectively and is known as fusion positiv...
ORGANISM(S): Human 
2020-11-20 | MSV000086494 | MassIVE
Genomicsα-Synuclein promotes H4R3me2s through interacting with BAF-PRMT5 complex
α-Synuclein promotes H4R3me2s through interacting with BAF-PRMT5 complex
PRJNA949282 | ENA
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