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Phosphate is essential for all living organisms, participating in critical biochemical processes, being an essential component of the energy dynamics of cells and being a component of nucleic acids, phospholipids in membranes, and other biomolecules. Therefore, the ability to withstand conditions of...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
Microarray were done on 4 independent cultures. PA01 was grown for 6 hours to late log growth phase (OD600 0.9-1.0) in liquid BM2-swarming media under shaking conditions(swimming) or for 18 hours at 37C on BM2-swarming plate containing 0.5% (w/v) agar and 0.1% casamino acids. Cells were harvested ...
ORGANISM(S): Pseudomonas aeruginosa 
5 Biological repeats of bacterial strain PAO1(parent, control) were compared to 5 biological repeats of PAO1_psrA::Tn5 knockout mutant, both strains were grown in BM2-glucose media containing 2mM MgSO4. Cells were grown to mid-log phase, OD600 0.50 Cells were immediately harvested and RNA was ext...
ORGANISM(S): Pseudomonas aeruginosa 
Five biological repeats of P.aeruginosa PAO1 in the absence of human LL37 and five biological repeats of P.aeruginosa presence of LL37 were grown in mineral medium in continuous-culture flow cells. Microarray experiments were done using microarray slides and protocols from TIGR on cells harvested f...
ORGANISM(S): Pseudomonas aeruginosa 
RNA was isolated from five biological repeats of P.aeruginosa and five biological repeats of P.aeruginosa parR mutant grown in BM2 media contianing glucose as the carbon source, 2 mM MgSO4 and 4 ug/ml indolicidin. Microarray experiments were done using microarray slides and protocols from http...
ORGANISM(S): Pseudomonas aeruginosa 
Four biological repeats of P.aeruginosa PA14 and four biological repeats of P.aeruginosa PA14 metR::phoA were grown in BM2 swarming media plates containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, 0.1% (wt/vol) casamino acids and 0.5% agar. Microarray e...
ORGANISM(S): Pseudomonas aeruginosa 
Five biological repeats of P.aeruginosa PA14 and five biological repeats of P.aeruginosa PA14 metR::phoA were grown in liquid BM2 swarming media containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, and 0.1% (wt/vol) casamino acids. Microarray experiments ...
ORGANISM(S): Pseudomonas aeruginosa 
Microarray experiments were preformed comparing the effect of sub-inhibitory concentrations of ciprofloxacin on five independent cultures of PAO1 and the PAO1 Lon mutant
ORGANISM(S): Pseudomonas aeruginosa PAO1 
Five biological repeats of P.aeruginosa PAO1 and five biological repeats of P.aeruginosa PAO1 phoQ::xylE were grown in mineral medium BM2 using glucose as the carbon source and with 2mM MgSO4. Microarray experiments were done using microarray slides and protocols from TIGR on cells from mid-log pha...
ORGANISM(S): Pseudomonas aeruginosa 
Micoarrays were used to obtain gene expression in mid-log phase PAO1::cprR(PA3077) with the treatment of 12ug/ml CP28 vs mid-log phase PAO1 with the treatment of 12ug/ml CP28
ORGANISM(S): Pseudomonas aeruginosa PAO1 
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