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TranscriptomicsTranscription profiling of C. elegans small RNA cloning and array methods for reproducibility and quantitation
This experiment was for the purpose of testing our small RNA cloning and array methods for reproducibility and quantitation (see Axtell and Bartel). This experiment consists of four hybridizations of C. elegans small RNA. Hybridizations test a Cy3-labeled biological sample against a constant, synthe...
ORGANISM(S): synthetic DNA 
2005-05-01 | E-MEXP-291 | biostudies-arrayexpress
TranscriptomicsMaternal and paternal genomes contribute equally to the transcriptome of early plant embryos
In animals, maternal gene products deposited into eggs regulate embryonic development before activation of the zygotic genome. In plants, an analogous period of prolonged maternal control over embryogenesis is thought to occur based on gene-expression studies. However, other gene-expression studies ...
ORGANISM(S): Arabidopsis thaliana 
2012-01-12 | E-GEOD-33713 | biostudies-arrayexpress
TranscriptomicsTranscript profiling of wild-type and dcl1-5 early globular embryos
DICER-LIKE1 (DCL1) is required for miRNA biogenesis and embryonic pattern formation. Presumably the patterning defects observed in dcl1-5 null embryos are due to the loss of miRNAs and the consequent up-regulation of their respective targets. To test which miRNA targets were up-regulated in dcl1 emb...
ORGANISM(S): Arabidopsis thaliana 
2010-12-01 | E-GEOD-25404 | biostudies-arrayexpress
TranscriptomicsArabidopsis microRNA Organ Map
This experiment was designed to determine the presence and levels of microRNAs and other select small RNAs in the major organs of wild-type (Col-0) Arabidopsis thaliana grown under standard conditions.



To obtain a global overview of miRNA levels in wild-type Arabidopsis, a tissue ex...
ORGANISM(S): Arabidopsis thaliana 
2005-05-01 | E-MEXP-292 | biostudies-arrayexpress
TranscriptomicsEndogenous shRNAs, siRNAs, and Other Microprocessor-independent, Dicer-dependent Small RNAs in Mouse ES Cells
Canonical microRNAs (miRNAs) require two processing steps: the first by the Microprocessor, a complex of DGCR8 and Drosha, and the second by Dicer. dgcr8delta/delta mouse embryonic stem cells (mESCs) have less severe phenotypes than dicer1delta/delta mESCs, suggesting a physiological role for Microp...
ORGANISM(S): Mus musculus 
2008-09-09 | E-GEOD-12521 | biostudies-arrayexpress
TranscriptomicsPhylogenetic Survey of Land Plant microRNAs
This experiment probed for the presence of known Arabidopsis and rice microRNAs in total RNA samples derived from species representative of the major groups of land plants: Eudicots (Arabidopsis thaliana, Nicotiana benthamiana), monocots (Oryza satica, Triticum aestivum), magnoliids (Liriodendron tu...
ORGANISM(S): Triticum aestivum 
2005-05-01 | E-MEXP-293 | biostudies-arrayexpress
ProteomicsProteomic investigation uncovers potential targets and target sites of pneumococcal serine-threonine kinase StkP and phosphatase PhpP
Like eukaryotes, different bacterial species express one or more Ser/Thr kinases and phosphatases that operate in various signaling networks by catalyzing phosphorylation and dephosphorylation of proteins that can immediately regulate biochemical pathways by altering protein function. The human path...
ORGANISM(S): Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) 
2020-01-22 | PXD015268 | Pride
Mass spectrometry Ser/thr kinases Label-free quantification Phosphopeptide enrichment Phosphoproteomics Spectral library Phosphatases Streptococcus pneumoniae 
UnknownSCAI Publications Committee Manual of Standard Operating Procedures: 2022 Update.
Not available
S-EPMC11307643 | biostudies-literature
UnknownGuidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Not available
S-EPMC4835977 | biostudies-literature
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