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ProteomicsLoss of centrosomal gene ALMS1 alters lipid metabolism and the regulation of processes linked to the extracellular matrix.
In this project we evaluated the proteomic profiling with TGF-β stimuli at 24h in a CRISPR-Cas9 model for ALMS1 gene in hTERT-BJ-5ta cells. Proteomic results showed a majority inhibition of downstream regulated pathways by the TGF-β, associating the protein coding genes (PCG) with processes like TGF...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD035708 | Pride
Tgf-β Akt Ciliopathy Alms1 Alström syndrome Lc-esi-ms/ms Primary cilia 
ProteomicsPhosphoproteomic profiling highlights CDC42 and CDK2 as key players in the regulation of the TGF-β pathway in ALMS1 and BBS1.
Using genomic editing with CRISPR-CAS9 and phosphoproteomics we have studied the TGF-β signalling pathway in knock-out (KO) models for ALMS1 and BBS1 genes. We have developed a network diffusion-based analysis pipeline to expand the data initially obtained and to be able to determine which processes...
ORGANISM(S): Homo sapiens (Human) 
2025-11-17 | PXD037573 | Pride
Ciliopathies Tgf-β Alms1 Bardet-biedl syndrome. Alström syndrome Phosphoproteomics Bbs1 
ProteomicsCRISPR-Cas9-mediated ALMS1 knockout inhibits TGF-β signalling and epithelial-mesenchyme transition.
In this project we evaluated the proteomic profiling with TGF-β stimuli at 24h in a CRISPR-Cas9 model for ALMS1 gene in HeLa cells. Proteomic results showed a majority inhibition of downstream regulated pathways by the TGF-β, associating the protein coding genes (PCG) with processes like focal adhes...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD024964 | Pride
Epithelial-mesenchyme transition Tgf-β Alms1 Hela Fibrosis Alström syndrome Lc-esi-ms/ms 
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