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ProteomicsYield and immunomodulatory effects of pneumococcal membrane vesicles change with bacterial growth phase
We investigated whether isolation of MVs during either the stationary-phase (24 h) or death-phase (48 h) affected their yield, immunogenicity and cytotoxicity in primary human immune cells. Death-phase vesicles showed higher yield than stationary-phase vesicles. Both vesicle types displayed excellen...
ORGANISM(S): Streptococcus pneumoniae (strain ATCC BAA-255 / R6) 
2022-02-16 | PXD026462 | Pride
Proteomics Primary macrophages Death-phase Stationary-phase Bacterial membrane vesicles Vaccine Immunogenicity 
ProteomicsEffect of iron-limitation, elevated temperature and florfenicol antibiotics on the subcellular proteomes and the vesiculation of the fish pathogen Aeromonas salmonicida
Here we analyzed the proteomic response of the important Gram-negative fish pathogen A. salmonicida to iron-limitation, the antibiotic florfenicol and an elevated incubation temperature. Proteins of different subcellular fractions (cytosol, inner membrane, outer membrane, extracellular and outer mem...
ORGANISM(S): Aeromonas salmonicida salmonicida 
2022-08-30 | PXD034641 | Pride
Proteomics Outer membrane vesicles Antibiotic Aeromonas salmonicida  iron limitation  temperature Florfenicol Subcellular fractionation 
ProteomicsProteome analysis of Renibacterium salmoninarum reveals putative role of membrane vesicles in virulence
This study used subcellular fractionation combined with proteomics for a comprehensive overview of the protein localizations of R. salmoninarum proteins within enriched subcellular fractions of cytosolic, membrane, extracellular and membrane vesicle (MV) proteins. With this approach, 1,674 protein g...
ORGANISM(S): Renibacterium salmoninarum ATCC 33209 
2022-02-24 | PXD025586 | Pride
Microbiology Membrane vesicles Proteomics Mass spectrometry Renibacterium salmoninarum 
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