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MetabolomicsRevealing the constituents of Egypt’s oldest beer using infrared and mass spectrometry
Previous studies have shown that the Ancient Egyptians used malted wheat and barley as the main ingredients in beer brewing, but the chemical determination of the exact recipe is still lacking. To investigate the constituents of ancient beer, we conducted a detailed IR and GC-MS based metabolite ana...
2020-01-06 | MTBLS1302 | MetaboLights
ProteomicsLabel-free shotgun proteomics of M. hyopneumoniae and M. feriruminatoris
Mycoplasmas are wall-less parasitic bacteria possessing extremely small genomes. Despite this, growth rates differ among mycoplasma species with doubling times ranging from only 0.5h to 16h. Here, we analyzed the whole proteomes of M. hyopneumoniae and M. feriruminatoris and performed a comparative ...
ORGANISM(S): Mycoplasma hyopneumoniae (strain 232) Mycoplasma feriruminatoris 
2021-08-23 | PXD025422 | Pride
M. hyopneumoniae M. feriruminatoris 
TranscriptomicsDistribution of Arp4 in G2/M phase
The actin-related proteins (ARPs) comprise a conserved protein family. Arp4p is found in large multisubunits of the INO80 and SWR1 chromatin remodeling complexes and in the NuA4 histone acetyltransferase complex. Here we show that arp4 (arp4S23AD159A) temperature-sensitive cells are defective in G2/...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-11 | E-GEOD-7276 | biostudies-arrayexpress
ProteomicsCharacterizing Peptidase Activity of Barley Endoprotease B in Complex Technical Matrices of the Beer Brewing Process
Barley cysteine endoprotease B (HvEPB) is an important enzyme in plant biology and food production. Pilot-scale fed-batch fermentation with Komagataella phaffii in minimal FM22 medium enabled controlled recombinant HvEPB (r-HvEPB) secretion. Peptidomics across four technological brewing matrices - r...
ORGANISM(S): Komagataella phaffii Hordeum vulgare subsp. vulgare 
2026-07-28 | PXD067323 | Pride
Beer Endoprotease b Epb Proteolysis Peptidases Gluten 
TranscriptomicsMetagenomic, metatranscriptomic and metaproteomic study of enrichment culture of M. oxyfera (denitrifying methanotroph)
DNA, RNA and protein were extracted from the culture and subjected to massive parallel sequencing and nano-LC-MS-MS respectively Combination of these methods enabled the reconstruction of the complete genome sequence of M oxyfera from the metagenome and identification of the functionally relevant en...
ORGANISM(S): Candidatus Methylomirabilis oxyfera 
2010-03-25 | E-GEOD-18535 | biostudies-arrayexpress
TranscriptomicsRNA at 92 M-BM-0C: the non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi.
The non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi is investigated using the RNA-seq technology. A dedicated computational pipeline analyzes RNA-seq reads and prior genome annotation to identify small RNAs, untranslated regions of mRNAs, and cis-encoded antisense transc...
ORGANISM(S): Pyrococcus abyssi 
2014-05-31 | E-GEOD-58131 | biostudies-arrayexpress
TranscriptomicsCo-inoculation with Dekkerea bruxellensis impacts the trancriptome of Saccharomyces cerevisiae during wine fermentation
The yeast Dekkera bruxellensis is as ethanol tolerant as Saccharomyces cerevisiae and may be found in bottled wine. It causes the spoilage of wine, beer, cider and soft drinks. In wines, the metabolic products responsible for spoilage by Dekkera bruxellensis are mainly volatile phenols. These chemic...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-12 | E-GEOD-38582 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of bottom-fermenting yeast Saccharomyces pastorianus KBY011 during a time series of lager beer fermentation in the wort
A transcriptome analysis of the bottom-fermenting yeast S. pastorianus KBY011 during a time course of lager beer fermentation in the wort was carried out (0, 2, 6, 8, 10, 14, 24, 34, 40, 52, 64, 120, and 156h). RNA preparation followed by DNA microarray analysis was performed for the yeast harveste...
ORGANISM(S): Saccharomyces pastorianus 
2007-12-31 | E-MEXP-1039 | biostudies-arrayexpress
TranscriptomicsIdentiM-oM-,M-^Acation of genes responsible for biosynthesis of kojic acid in Aspergillus oryzae
Kojic acid is produced in large amounts by Aspergillus oryzae as a secondary metabolite and is widely used in the cosmetic industry. Glucose can be converted to kojic acid, perhaps by only a few steps, but no genes for the conversion have thus far been revealed. Using a DNA microarray, gene expressi...
ORGANISM(S): Aspergillus oryzae 
2013-12-07 | E-GEOD-43280 | biostudies-arrayexpress
TranscriptomicsInfluence of 17M-NM-2-estradiol on Gene Expression of Paracoccidioides During Mycelia-to-Yeast Transition
Background: Paracoccidioides is the causative agent of paracoccidioidomycosis, a systemic mycosis endemic to Latin America. Infection is initiated by inhalation of conidia (C) or mycelial (M) fragments, which subsequently differentiate into yeast (Y). Epidemiological studies show a striking predomin...
ORGANISM(S): Paracoccidioides brasiliensis Pb01 
2011-12-14 | E-GEOD-30466 | biostudies-arrayexpress
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