Sort   by:  
 Page size 
TranscriptomicsIdentification of chicken Interferon Stimulated Genes (ISGs) using Affymetrix Chicken Gene 1.0 ST Arrays
Recombinant chicken IFN1 was prepared as previously reported (Laidlaw et al, 2013) and was added in chicken embryo fibroblasts to a final concentration of 1000 U/ml. Confluent cells were treated with chicken IFNα or mock treated and incubated for six hours before harvesting. The experiment was repea...
ORGANISM(S): Gallus gallus 
2015-12-01 | E-MTAB-3712 | biostudies-arrayexpress
TranscriptomicsIdentification of chicken Interferon Stimulated Genes (ISGs) using Affymetrix GeneChip Chicken Genome Arrays
Recombinant chicken IFN1 was prepared as previously reported (Laidlaw et al, 2013) and was added in chicken embryo fibroblasts to a final concentration of 1000 U/ml. Confluent cells were treated with chicken IFNa or mock treated and incubated for six hours before harvesting. The experiment was repea...
ORGANISM(S): Gallus gallus 
2015-12-01 | E-MTAB-3711 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of Fowlpox knock-out mutant viruses using Affymetrix GeneChip Chicken Genome Arrays
Fowlpox virus (FWPV) is a double-stranded DNA virus, used as a live vaccine against poultry diseases, and considered a promising potential mammalian vaccine vector. Similar to mammalian poxviruses, FWPV has evolved mechanisms to evade host immune responses at different levels. We infected chicken em...
ORGANISM(S): Gallus gallus 
2019-01-01 | E-MTAB-7276 | biostudies-arrayexpress
TranscriptomicsKinetics of host transcription in CEFs productively infected with recombinant fowlpox virus vaccine vector FP9
Fowlpox virus (here as FP9, a plaque-purified, high passage-attenuated derivative) effectively suppresses induction of the ‘innate’ immune responses, notably the Type I interferon system, of the permissive host (chicken). Despite the extensive usage of fowlpox virus as a recombinant vaccine vect...
ORGANISM(S): Gallus gallus 
2018-12-31 | E-MTAB-5455 | biostudies-arrayexpress
TranscriptomicsGenome-wide comparison of Chicken Embryo Fibroblasts (CEF) and DF-1 in response to recombinant chicken chIFN-α and infection with Infectious Bursal Disease Virus (IBDV)
The spontaneously immortalised chicken DF-1 cell line is rapidly replacing its progenitor primary chicken embryo fibroblasts (CEF) in studies on avian viruses but no comprehensive study has as yet been reported comparing their immune phenotype. We conducted microarray analysis of the DF-1 and CEF, i...
ORGANISM(S): Gallus gallus 
2016-01-15 | E-MTAB-4028 | biostudies-arrayexpress
Sort   by:  
 Page size