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MetabolomicsLC-HRMS Metabolomics for Untargeted Diagnostic Screening in Clinical Laboratories: A Feasibility Study
Today's high-resolution mass spectrometers (HRMS) allow bioanalysts to perform untargeted/global determinations that can reveal unexpected compounds or concentrations in a patient’s sample. This could be performed for preliminary diagnosis attempts when usual diagnostic processes and targeted ...
2019-06-06 | MTBLS476 | MetaboLights
ProteomicsCo-translational determination of quaternary structures in chaperone factories
The HSP90/R2TP quaternary chaperone assembles key cellular machines, including the three nuclear RNA polymerases and many non-coding RNPs. Here, we show that the R2TP binds many partners co-translationally. Remarkably, its co-translational interactome reveals many novel potential clients, and side-b...
ORGANISM(S): Homo sapiens (Human) 
2026-01-06 | PXD061298 | Pride
Translation factories Chaperone Co-translational assembly 
ProteomicsInteractome of NOP58 WT vs A283P mutant
The PAQosome is composed of the HSP90/R2TP chaperone and a prefoldin-like module. It promotes the biogenesis of cellular machineries but it is unclear how it discriminates closely related client proteins. Among the main PAQosome clients are C/D snoRNPs and in particular their core protein NOP58. Usi...
ORGANISM(S): Homo sapiens (Human) 
2020-12-14 | PXD019414 | Pride
Apms  lc-msms  silac 
ProteomicsInteractome of NOP58 WT vs KA_AR mutant
The PAQosome is composed of the HSP90/R2TP chaperone and a prefoldin-like module. It promotes the biogenesis of cellular machineries but it is unclear how it discriminates closely related client proteins. Among the main PAQosome clients are C/D snoRNPs and in particular their core protein NOP58. Usi...
ORGANISM(S): Homo sapiens (Human) 
2020-12-14 | PXD019415 | Pride
Apms  silac  lc-msms 
ProteomicsInteractome of Prpf31 WT vs KA_AR mutant
The PAQosome is composed of the HSP90/R2TP chaperone and a prefoldin-like module. It promotes the biogenesis of cellular machineries but it is unclear how it discriminates closely related client proteins. Among the main PAQosome clients are C/D snoRNPs and in particular their core protein NOP58. Usi...
ORGANISM(S): Homo sapiens (Human) 
2020-12-14 | PXD019416 | Pride
Apms  silac  lc-msms 
ProteomicsInteractome of Prpf31 Wild-type and A216P
The PAQosome is composed of the HSP90/R2TP chaperone and a prefoldin-like module. It promotes the biogenesis of cellular machineries but it is unclear how it discriminates closely related client proteins. Among the main PAQosome clients are C/D snoRNPs and in particular their core protein NOP58. Usi...
ORGANISM(S): Homo sapiens (Human) 
2020-12-14 | PXD019390 | Pride
Apms  prpf31  silac 
ProteomicsInteractome of HNTG c12orf45 protein
The PAQosome is composed of the HSP90/R2TP chaperone and a prefoldin-like module. It promotes the biogenesis of cellular machineries but it is unclear how it discriminates closely related client proteins. Among the main PAQosome clients are C/D snoRNPs and in particular their core protein NOP58. Usi...
ORGANISM(S): Homo sapiens (Human) 
2020-12-14 | PXD019389 | Pride
Hela cells  apms  lc-msms 
ProteomicsSILAC APMS analysis of Pih1D2 interactome
To get insights into the function of Pih1D2 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-Pih1D...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009520 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of DNAAF2 interactome
To get insights into the function of DNAAF2 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP- DNAA...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009501 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of RPAP3 C-term interactome
To get insights into the function of RPAP3, we characterized its partners by performing a proteomic analysis in human cells. We fused the Cterm part of RPAP3 domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009518 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
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