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ProteomicsThe proteins interacting with GST-betaTub85D from the GST pulldown
First, GST-betaTub85D and GST proteins are expressed, followed by pulldown experiments with glutathione sepharose, and finally, mass spectrometry is used to identify the captured proteins.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-09-29 | PXD056093 | Pride
Betatub85d Wolbachia Drosophila 
TranscriptomicsTranscription profiling of mouse B6 and tub/tub mice strains muscle tissues
This experiement was done to compare gene expression in muscular tissue in two different strains. The experiment was a 6 slide loop dye-flip experiment using muscle RNA from 3 biological replicates for B6 and tub/tub mice strains.
ORGANISM(S): Mus musculus 
2003-12-16 | E-MEXP-47 | biostudies-arrayexpress
TranscriptomicsKlf5 controls bone marrow homing of stem cells and progenitors through Rab5-mediated membrane Beta1/Beta2-integrin expression
The Kruppel-like factor 5 (Klf5) regulates pluripotent stem cell self-renewal but its role in somatic stem cells is unknown. Klf5 deficient hematopoietic stem cells and progenitors (HSC/P) fail to engraft after transplantation. This HSC/P defect was associated with impaired bone marrow (BM) homing a...
ORGANISM(S): Mus musculus 
2013-01-30 | E-GEOD-43806 | biostudies-arrayexpress
TranscriptomicsGenes regulated by ectopic expression of Sage in the entire Drosophila embryo using RNA from tub-Gal4 embryos.
FoxA transcription factors play major roles in organ-specific gene expression. How FoxA proteins achieve specificity is unclear, given their broad expression patterns and requirements in multiple cell types. Here, we characterize Sage, a basic helix-loop-helix (bHLH) transcription factor expressed e...
ORGANISM(S): Drosophila melanogaster 
2013-12-31 | E-GEOD-44090 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse duodenum and liver with primary (Hfe ?/? and C282Y homozygous mice) and secondary iron overload vs 129S6/SvEvTac wild type controls
We determined duodenal and liver gene response patterns in mice with primary (Hfe ?/? and C282Y homozygous mice) and secondary iron overload versus 129S6/SvEvTac wild type controls.
ORGANISM(S): Mus musculus 
2013-01-30 | E-MEXP-5 | biostudies-arrayexpress
TranscriptomicsExpression data from Bone-Marrow Mesenchymal Stromal Cells (BM-MSC) from Follicular Lymphoma patient (FL) or Healthy Donor (HD).
Human bone marrow mesenchymal stromal cells (BM-MSC) could be committed toward a functional lymphoid-like stroma by a combination of TNFalpha (TNF) and Lymphotoxin alpha1/beta2 (LT) (Amé-Thomas et al Blood 2007). Bone marrow and lymph node stromal cells support FL malignant cell recruitment and grow...
ORGANISM(S): Homo sapiens 
2012-01-26 | E-GEOD-35331 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human hematopoietic stem cells engrafted into fetal goats
Robust human-goat chimerism was achieved by transplanting human CD34+Lin- cord blood cells into fetal goats. We observed a broad distribution of GFP-marked human cells in non-hematopoietic organs including kidney, muscle, lung, and heart of transplant goats. Various marker techniques indicated that...
ORGANISM(S): Homo sapiens 
2007-07-19 | E-GEOD-1801 | biostudies-arrayexpress
GenomicsGenome wide mapping of long noncoding (lnc) RNAs in hepatic stellate cells
Hepatic stellate cells are the primary cell type responsible for development of fibrosis in chronic liver disease. We used directional RNA sequencing (RNA-seq) and chromatin immunoprecipitation and sequencing (ChIP-seq) to identify the lncRNAs expressed in human HSCs. We also identified the lncRNAs...
ORGANISM(S): Homo sapiens 
2016-03-23 | E-GEOD-68108 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human trabecular meshwork cells after TGF-beta1 and 2 treatment
TGF-beta levels are known to increase in the aqueous humor of eye cells in patients with glaucoma. Increase TGF-beta is assumed to have a biochemical impact on the trabecular meshwork, and an increase in extracellular matrix formation, which may be responsible for decrease outflow facility of the e...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7144 | biostudies-arrayexpress
GenomicsTOP1M-NM-1 functions on nucleosome density
DNA topoisomerases release supercoils in DNA introduced during replication or transcription. How DNA topoisomerases impact transcription in the context of eukaryotic chromatin is poorly understood. In this study, using a floral stem cell model in Arabidopsis, we uncovered a role of TOP1M-NM-1 in Pol...
ORGANISM(S): Arabidopsis thaliana 
2014-10-14 | E-GEOD-53950 | biostudies-arrayexpress
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