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ProteomicsRobust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting
Micro-flow liquid chromatography tandem mass spectrometry (µLC–MS/MS) is increasingly becoming an alternative to nano-LC–MS/MS for the analysis of proteomes. We have recently demonstrated the potential of a µLC–MS/MS system operating with a 1 mm i.d. × 150 mm column and at a flow rate of 50 µl/min f...
ORGANISM(S): Homo sapiens (Human) 
2021-02-22 | PXD023650 | Pride
Micro-flow lc Plasma Proteomics Urine 1.0 mm id column 
ProteomicsMicro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Homo sapiens (Human) 
2021-07-01 | PXD023587 | Pride
Single shot proteomics Micro-flow lc 1.0 mm id column 
ProteomicsHigh-flow chromatography for reproducible and high-throughput quantification of proteomes
This study evaluates liquid-chromatography operated at 50 ul/min using 1 mm diameter columns coupled online to tandem mass spectrometry (LC-MS/MS) for proteome analysis. Data from >1,000 human cell line, tissue, body fluid, affinity- and phospho-proteomes demonstrate excellent chromatographic (<0.3 ...
ORGANISM(S): Homo sapiens (Human) 
2019-12-09 | PXD015087 | Pride
High-flow lc Chromatography Proteomics Body fluids Kinobeads 
ProteomicsComparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD024169 | Pride
Single shot proteomics Nano-flow lc Micro-flow lc 
ProteomicsHs_Hela_Phospho_Gluc
Hs Hela Cell, Ti4+-IMAC phospho enrichment, Gluc digest
ORGANISM(S): Homo Sapiens (human) 
2012-12-31 | PAe005275 | PeptideAtlas
ProteomicsHs_Hela_Phospho_Trypsin
Hs Hela Cell, Ti4+-IMAC phospho enrichment, Trypsin digest
ORGANISM(S): Homo Sapiens (human) 
2012-12-31 | PAe005251 | PeptideAtlas
ProteomicsMass spectrometry-based draft of the mouse proteome
Quantitative draft map of the proteomes of 41 healthy tissues of the animal model Mus musculus and a panel of 66 murine pancreatic ductal adenocarcinoma cell lines and. Our in-depth analysis provides evidence for proteins encoded by >16,000 genes (~76% of the total annotated protein-coding genes), w...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-07-09 | MSV000089856 | MassIVE
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