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ProteomicsDifferential kinobeads selectivity profiling
The goal of this project was to use differential kinobeads profiling for the discrimination of reversible and irreversible kinase targets of different covalent kinase inhibitors. For this, differences in target engagement measured in a lysate- and a cell-based kinobeads assay were compared in a kino...
ORGANISM(S): Homo sapiens (Human) 
2017-10-13 | PXD007920 | Pride
Ibrutinib Kinobeads Ramos b-lymphocyte Cell-based assay 5z-7-oxozeaenol Saturated ibrutinib A549 lung epithelia cell line Lysate-based assay Acalabrutinib Cc-292 Lc-ms/ms 
ProteomicsComprehensive selectivity profile of PARP inhibitors using kinobeads
Explore kinase off-targets of several PARP inhibitors using kinobeads
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD045297 | Pride
Kinobeads Niraparib Parp Talazoparib Olaparib 
ProteomicsKinobeads affinity enrichment from lysate derived from stimulated cells
Investigate differential binding of kinases to Kinobeads depending on activation state
ORGANISM(S): Homo sapiens (Human) 
2018-05-02 | PXD008632 | Pride
Kinobeads Lysate-based assay Lc-ms/ms 
UnknownDevelopment of a novel AAK1 inhibitor via Kinobeads-based screening.
Not available
S-EPMC10954696 | biostudies-literature
ProteomicsPharmacoproteomic characterisation of human colon and rectal cancer - CRC65 Kinobeads
Most molecular cancer therapies act on protein targets but data on the proteome status of patients and cellular models are only beginning to emerge. Here, we profiled the proteomes of 65 colorectal cancer (CRC) cell lines to a depth of >10,000 proteins using mass spectrometry. Integration with prote...
ORGANISM(S): Homo sapiens (Human) 
2017-12-12 | PXD005355 | Pride
Personalised medicine Integrated full proteome subtypes Crc65 Mass spectrometry Correlation of mrna and protein abundance Patient stratification Consensus molecular subtypes Cptac Quasar2 Kinobeads 
ProteomicsChemoproteomic selectivity profiling of PIKK and PI3K kinase inhibitors
"Here, we report the development of a novel version of Kinobeads that extends kinome coverage to PIKK and PI3K kinases. This is achieved by inclusion of two affinity probes derived from the clinical PI3K/MTOR inhibitors Omipalisib and BGT226. The new affinity matrix was used to profile 13 clinical a...
ORGANISM(S): Homo sapiens (Human) 
2019-03-29 | PXD011719 | Pride
Chemoproteomics" "kinobeads Drug profiling 
ProteomicsAquired resistance mechanisms to EGFR-specific kinase inhibitors using chemical proteomics
Here we used kinase affinity enrichment (Kinobeads) and intensity-based mass spectrometry to elucidate aquired resistance mechanisms to EGFR inhibition in cancer cells. First, kinase expression differences between untreated control cells and long term treated resistant cells were investigated using ...
ORGANISM(S): Homo sapiens (Human) 
2015-05-20 | PXD001825 | Pride
Lc-ms/ms Cancer Kinase inhibitor Drug resistance 
ProteomicsHigh-performance proteomics using nano-, capillary- and micro-flow chromatographic separations
In this study, we systematically evaluated the performance of μLC (50, 10 μL/min), capLC (5, and 1.5 μL/min), and nLC (0.3 μL/min ) flow-rates for proteome analysis using a Vanquish Neo HPLC coupled with a Q Exactive HF-X mass spectrometer. Serial dilutions of HeLa cell line digests were used for b...
ORGANISM(S): Homo sapiens (Human) 
2025-09-03 | PXD062536 | Pride
Kinobeads Lc-ms Phospho-proteomics Capillary-flow chromatography 
ProteomicsTRIM24 project, kinobeads affinity profiles and cellular degradation profiles
This dataset contains kinobeads selectivity profile data for Kinase inhibitors and Kinase inhibitor based PROTACs along with data for the cellular perturbation of those molecules.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000095896 | MassIVE
ProteomicsCompetitive kinobead profiling of RAF inhibitors
Kinase enrichment was performed using kinobeads followed by LC–MS/MS to profile target engagement/competition by RAF inhibitors. HEK293T lysates overexpressing 185 protein kinases were prepared in modified RIPA buffer, pre-incubated with DMSO or inhibitor, then incubated with kinobeads to enrich kin...
ORGANISM(S): Homo sapiens (Human) 
2026-04-20 | PXD075247 | Pride
Human Lc-ms Kinase 
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