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ProteomicsGlycine receptor complex analysis using immunoprecipitation-blue native gel electrophoresis-mass spectrometry
The pentameric glycine receptor (GlyR), comprising the α1 and β subunits, is a major inhibitory ionotropic receptor in brainstem and spinal cord. GlyRs interact with gephyrin (GPHN), a scaffold protein that anchors the GlyR in the plasma membrane and enables it to form clusters in glycinergic postsy...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-12 | PXD017151 | Pride
Blue native gel electrophoresis Proteomics Protein complex Glycine receptor Iqsec3 
ProteomicsA new method for differential proteomic analysis utilizing native MS-electropherograms: a comparison between plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS
MS identification has long been used for PAGE-separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole-gel slicing and quantitative LC-MS/MS aiming c...
ORGANISM(S): Homo sapiens (Human) 
2017-09-08 | PXD006773 | Pride
Gel slicing Quantitative lc-ms/ms Native ms-electropherogram Native 1d-page Human plasma and serum Differential proteomic analysis 
ProteomicsBlue native gel electrophoresis of Syntrophus aciditrophicus proteome
Blue native gel electrophoresis of Syntrophus aciditrophicus proteome
ORGANISM(S): Syntrophus Aciditrophicus Sb 
2019-04-29 | PXD011101 | JPOST Repository
TranscriptomicsTranscription profiling of Arabidopsis wild type Col-0, cry1 and hfr1 plants in response to dark and blue light treatment
micrarray profiling on Arabidopsis thaliana col-0, cry1 and hfr1 in response to dark and 1 h blue light treatment
ORGANISM(S): Arabidopsis thaliana 
2008-10-29 | E-MEXP-1784 | biostudies-arrayexpress
TranscriptomicsTranscriptomic responses to salinity stress in invasive and native blue mussels (genus Mytilus)
The invasive marine mussel Mytilus galloprovincialis has displaced the native congener Mytilus trossulus from central and southern California, but the native species remains dominant at more northerly sites that have high levels of freshwater input. To determine the extent to which interspecific dif...
ORGANISM(S): Mytilus galloprovincialis 
2011-01-10 | E-GEOD-25111 | biostudies-arrayexpress
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009510 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsComplexome profiling of membrane fraction from Nox4 overexpressing HEK293 cells
Within the family of NADPH oxidases, Nox4 is unique as it is predominantly localized in the endoplasmic reticulum, has constitutive activity and generates H2O2. We hypothesize that these features are consequences of a so far unidentified Nox4-interacting protein. Blue native gel electrophoresis of ...
ORGANISM(S): Homo sapiens (Human) 
2017-04-07 | PXD003509 | Pride
Lc-msms Blue native electrophoresis Complexome profiling Macromolecular complexes 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009485 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsGlobal mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and label-free quantitative LC-MS/MS
Plasma samples from adult male rats were separated by nondenaturing micro 2DE and a representative gel was selected, on which a total of 136 CBB-stained spots were numbered and subjected to in-gel digestion and label-free quantitative LC-MSE (MS/MS in data-independent acquisition mode). The analysi...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-10-14 | PXD004395 | Pride
Native proteomics Adult male rat plasma proteome Nondenaturing micro 2de Global mapping Label-free quantitative lc-mse 
UnknownResolving mitochondrial protein complexes using nongradient blue native polyacrylamide gel electrophoresis.
Not available
S-EPMC2795571 | biostudies-literature
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