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TranscriptomicsChIP-chip by array of human cells for whole genome profiling of 4 histone modifications
Whole genome profiling of 4 histone modifications (H3K27me1, H3K27me3,H3K36me1 and H3K36me3) using ChIP-on-chip. It has recently been shown that nucleosome distribution, histone modifications and RNA polymerase II (Pol II) occupancy show preferential association with exons ("exon-intron marking"), l...
ORGANISM(S): Homo sapiens 
2010-12-03 | E-MTAB-336 | biostudies-arrayexpress
GenomicsQuantifying ChIP-seq data: a spiking method providing an internal reference for sample-to-sample normalization.
Purpose: Description of a spike-adjusting-method (SAM) to normalize ChIP-seq data . Methods: We performed ChIP-seq of POLR3D and POLR2B with mouse liver supplemented with 2.5% of human DNA. Human DNA will be used as an internal control for ChIP-seq quantification. Results: We show that using the SA...
ORGANISM(S): Mus musculus 
2014-04-02 | E-GEOD-52049 | biostudies-arrayexpress
TranscriptomicsChIP-chip by array of human K562, U937 and CD14+ cells to identify histone modifcations in ENCODE regions
Chromatin immunoprecipitation microarray (ChIP-chip) study using 3 human cell lines (K562, U937, CD14+); of 19 histone modifications across 1% of the human genome used in the pilot phase of the ENCODE project.
paper abstract: It has recently been shown that nucleosome distribution, histone modifi...
ORGANISM(S): Homo sapiens 
2010-12-29 | E-MTAB-334 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human cancer cell lines K562 and U937
Transcription profiling of two cancer cell lines: K562 and U937. It has recently been shown that nucleosome distribution, histone modifications and RNA polymerase II (Pol II) occupancy show preferential association with exons ("exon-intron marking"), linking chromatin structure and function to co- t...
ORGANISM(S): Homo sapiens 
2010-12-03 | E-MTAB-335 | biostudies-arrayexpress
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