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ProteomicsImproving identification of in-organello protein-protein interactions using an affinity-enrichable, isotopically-coded, and mass spectrometry-cleavable chemical crosslinker
An experimental and computational approach for identification of protein-protein interactions by in-vivo chemical crosslinking and mass spectrometry (CLMS) has been developed that takes advantage of the specific characteristics of cyanurbiotindipropionylsuccinimide (CBDPS), an affinity-tagged isotop...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-17 | PXD014055 | Pride
Cross-linking Yeast Mitochondria 
ProteomicsImproving identification of in-organello protein-protein interactions using an affinity-enrichable, isotopically-coded, and mass spectrometry-cleavable chemical crosslinker
An experimental and computational approach for identification of protein-protein interactions by in-vivo chemical crosslinking and mass spectrometry (CLMS) has been developed that takes advantage of the specific characteristics of cyanurbiotindipropionylsuccinimide (CBDPS), an affinity-tagged isotop...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-17 | PXD017066 | Pride
Cross-linking Yeast Mitochondria 
ProteomicsChaperone Activation and Client Binding of a 2-Cysteine Peroxiredoxin
Many 2-Cys-peroxiredoxins (2-Cys-Prxs) serve as dual-function proteins. Active as peroxidases under non-stress conditions, they convert into effective chaperones under stress conditions. While their peroxidase activity has been extensively studied and shown to involve cycles of redox-mediated oligom...
ORGANISM(S): Leishmania infantum Photinus pyralis (Common eastern firefly) (Lampyris pyralis) 
2019-02-19 | PXD010281 | Pride
Cross-linking  chaperone activation  chaperone-client complex 
ProteomicsConcentration determination of >200 proteins in dried blood spots for biomarker discovery and validation
Raw files were created using XCalibur 3.0.63 (Thermo Scientific) software and analysed with Thermo Proteome Discoverer 2.2.0.388 software suite (Thermo Scientific). The raw data files were processed as previously described 26 with the following modifications: the peak lists were submitted to Mascot...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-27 | MSV000084629 | MassIVE
MetabolomicsSequential multi-omics analysis identifies clinical phenotypes and predictive biomarkers for long COVID

The post-acute sequelae of COVID-19 (PASC), also known as long COVID, is often associated with debilitating symptoms and adverse multisystem consequences. We obtain plasma samples from 117 individuals during and 6 mth following their acute phase of infection to comprehensively profile and as...

2024-02-23 | MTBLS7337 | MetaboLights
UnknownMultiple Reaction Monitoring Enables Precise Quantification of 97 Proteins in Dried Blood Spots.
Not available
S-EPMC4638049 | biostudies-literature
ProteomicsCircadian Control of the Response of Macrophages to Plasmodium spp.-Infected Red Blood Cells.
In this study, we sought to determine whether the intensity of the immune response to Plasmodium spp., the parasite causing malaria, depends on time of infection. For this, we considered the known existence of a circadian clock in immune cells which regulates several aspects of the immune response. ...
ORGANISM(S): Mus musculus (Mouse) 
2024-06-26 | PXD049209 | Pride
Immune responses. Circadian rhythms Infected red blood cells Macrophages Plasmodium spp. 
ProteomicsSysQuan: repurposing SILAC mice for affordable absolute quantitation of the human proteome
Relative quantitation, used by most MS-based proteomics laboratories to determine protein fold-changes, requires that samples are processed and analyzed together to enable best comparability through minimizing batch differences. This limits the detection of subtle but relevant changes in heterogeneo...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-06-16 | PXD057266 | Pride
Proteomics Silac Stable isotope Targeted proteomics Absolute quantitation 
ProteomicsIRP1 deficiency triggers metabolic reprograming, increases insulin sensitivity and protects mice against metabolic syndrome pathologies
Iron regulatory protein 1 (IRP1) is a post-transcriptional regulator of cellular iron metabolism. Irp1-/- mice are viable but develop polycythemia. We report that these animals also exhibit fasting hypoglycemia and are protected against high fat diet-induced hyperglycemia and liver steatosis. Mechan...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-17 | PXD051897 | Pride
Proteomics Irp1 Liver Mouse Insulin 
ProteomicsLigand-induced disorder-to-order transitions characterized by structural proteomics and molecular dynamics simulations
We have studied both the Spy and the FKBP protein-ligand systems by structural proteomics and molecular -dynamics simulations. We have used hydrogen/deuterium exchange, differential crosslinking, and surface modification to characterize the conformational changes that occur upon both peptide bindin...
ORGANISM(S): Escherichia coli 
2021-09-08 | PXD015275 | Pride
Structural proteomics  mass spectrometry  discrete molecular dynamics simulations  protein-ligand interaction  hydrogen/deuterium exchange  crosslinking/mass spectrometry  surface modification  conformational change  quantitative protein structure  intrinsically disordered protein  protein folding 
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