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Parental dietary conditions can influence the metabolic traits of offspring. In mice, paternal consumption of low protein diet alters cholesterol and lipid metabolism of progeny. Here, we examine RNA species expressed in male reproductive tissues of mice. Protein restriction leads to altered levels ...
ORGANISM(S): Mus musculus 
RNA-seq of single mouse embryos using SMART-seq2. The embryos have been generated with IVF and are staged as hours post-IVF. At the zygote stage (~ 4h post-IVF), the embryos have been microinjected with 1.7 μg/μL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Non-injected embryos are denoted as...
ORGANISM(S): Mus musculus 
RNA-seq of single Fully Grown Oocytes using SMART-seq2. The oocytes were collected from female FVB mice, approximately. 44-48 h post-PMSG injection. The oocytes were microinjected with 1.3-1.7 μg/μL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Noninjected oocytes are denoted as “Noninj”. The ...
ORGANISM(S): Mus musculus 
Single-embryo SMART-seq2 of mouse 2-cell stage embryos. The embryos have been generated with in vitro fertilization (IVF) and are staged as hours post-IVF.
ORGANISM(S): Mus musculus 
Single-embryo SMART-seq of mouse Embryonic Genome Activation with 2-cell stage embryos collected after 2 h precision in vitro fertilization (IVF) at the following timepoints: 17, 20, 23, and 26 h post-IVF. Individual embryos were lysed in total cell lysis buffer, and the lysates were split to perfor...
ORGANISM(S): Mus musculus 
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