Tagged AKT2 was expressed in HEK293T cells. For quantification SILAC labeling was performed. MBP-TAP: Cell lysates were mixed at a 1:1 ratio with unlabeled wild type cells before Tandem Affinity Purification. MAP-TAP: Same amount of labeled and unlabeled cell lysates were purified via Tandem Affinit...
ORGANISM(S): Homo sapiens (Human)
