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LC-MS/MS analysis of Split-BirA expressed in mouse Astrocytes. Data aquired on a Fusion Lumos and subjected to label-free relative quantitation withing Protein Discoverer (v 4.2). Sample key; Split-TuboID (47826, 47827, 47825) soluble TurboID (47838, 47839, 47840) TurboID-surface (TurboID-GPI; 478...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-07-23 | MSV000085821 | MassIVE
Autophagy is essential for protein quality control and regulation of the functional proteome. Failure of autophagy pathways with age contributes to loss of proteostasis in aged organisms and accelerates progression of age-related diseases. In this work, we show that activity of endosomal microautoph...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-11 | PXD035128 | Pride
As systems biology approaches to virology have become more tractable, it has become possible to analyze highly studied viruses such as HIV in new, unbiased ways, including spatial proteomics. We have employed here a differential centrifugation protocol to fractionate an inducible model of HIV-expres...
ORGANISM(S): Homo sapiens (Human) 
2022-01-16 | PXD029956 | Pride
The interaction between HIV-1 and the host cell has been studied at many levels, but cell-wide organellar changes caused by the virus have not been defined. Here, we aimed to detail perturbations to the host cell spatial proteome following HIV protein expression. Subcellular fractionation followed b...
ORGANISM(S): Homo sapiens (Human) 
2022-01-28 | PXD024716 | Pride
Comparative analysis of the transcriptome of primary tumors generated from 4T1 cells transduced with a lentiviral vector expressing a siRNA against murine SPARC (4T1-C18), primary tumors generated from 4T1 cells transduced with a lentiviral vector expressing a scramble sequence (4T1-SCR) or lung met...
ORGANISM(S): Mus musculus 
Comparative analysis of the transcriptome of 4T1 cells stably transduced with a lentiviral vector expressing a siRNA against murine SPARC (4T1-C18) with 4T1 control cells stably transduced with a lentiviral vector expressing a scramble sequence (4T1-SCR). Two-condition experiment, 4T1-C18 vs. 4T1-SC...
ORGANISM(S): Mus musculus 
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