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The Drosophila melanogaster multi zinc finger protein hangover (hang) regulates responses to oxidative stress, heat shock and ethanol exposure by modulating transcript levels in adult fly heads. The hangover protein contains no less than 18 C2H2-type zinc fingers. These domains are prevalent among c...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2025-12-22 | PXD062946 | Pride
Affinity purification of dCoREST-interacting proteins from Drosophila melanogaster S2 cell nuclear extracts. An antibody recognizing both dCoREST isoforms was used to affinity purify proteins interacting with endogenous dCoREST from S2 nuclear extract. In addition, nuclear extracts from S2 cell line...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-11-11 | PXD014857 | Pride
Drosophila melanogaster KC cells were treated with RNAi against dL(3)mbt, dLint1 or EGFP (control) and the resulting changes in gene expression were measured.
ORGANISM(S): Drosophila melanogaster 
By combining extensive biochemical fractionation with quantitative mass spectrometry, we directly examined the composition of soluble multiprotein complexes among diverse animal models. The project has been jointly supervised by Andrew Emili and Edward M. Marcotte. Project website: http://metazoa.m...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2015-06-17 | PXD002321 | Pride
Expression of dCoREST or dLSD1 was reduced by RNAi in Drosophila testes and changes to gene transcription were determined by RNA-seq.
ORGANISM(S): Drosophila melanogaster 
We employed CRISPR/Cas-mediated genome editing to generate S2 cell lines expressing GFP-tagged dCoREST, dL(3)mbt, dLSD1 and dG9a, respectively. This allowed us to determine the genome-wide binding profiles for these proteins by ChIP-seq using the same antibody (anti-GFP) in each case.
ORGANISM(S): Drosophila melanogaster 
RNAi KD of dCoREST, dLSD1, dL(3)mbt, dLint-1, dG9a and EGFP (control) followed by determination of gene transcription changes by RNA-seq
ORGANISM(S): Drosophila melanogaster 
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