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ProteomicsMouse psd95 coimmunoprecipitation from excitatory or inhibitory neurons and mass spectrometry
We used a psd95-GFP cre dependent KI mouse to label PSD95 with GFP specifically in excitatory (CaMK) or inhibitory (vGAT) neurons. PSD95 was pulled down with a GFP nanobody and the resulting complex was used for massspectrometry analysis.
ORGANISM(S): Mus musculus (Mouse) 
2023-06-03 | PXD041635 | Pride
Synapse Mouse Psd95 Co-ip 
ProteomicsSex and experience dependent regulation of synaptic protein turnover
Synaptic transmission can be carefully tuned through plasticity mechanisms that regulate synaptic strength, structure, and number. In vivo measurements demonstrate remarkable spine dynamics, with subsets of synapses persisting for months. This correlates with the extreme longevity of certain memorie...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-07 | PXD065808 | Pride
Silam Protein turnover Lc-ms/ms 
UnknownPICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin.
Not available
S-EPMC5626541 | biostudies-literature
ProteomicsExperience-induced remodeling of the hippocampal post-synaptic proteome and phosphoproteome
The postsynaptic density (PSD) of excitatory synapses contains a highly organized protein network with thousands of proteins and is key node in the regulation of synaptic plasticity. To gain new mechanistic insight into experience-induced changes in the PSD, we examined the global dynamics of the hi...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-26 | PXD045496 | Pride
Proteomics Mouse Phosphoproteomics 
UnknownBtbd11 supports cell-type-specific synaptic function.
Not available
S-EPMC10592477 | biostudies-literature
UnknownCell-specific regulation of gene expression using splicing-dependent frameshifting.
Not available
S-EPMC9526712 | biostudies-literature
UnknownReport on the sixth blind test of organic crystal structure prediction methods.
Not available
S-EPMC4971545 | biostudies-literature
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