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ProteomicsMultiparameter functional diversity of human C2H2 zinc finger proteins
We have identified protein protein interactions of 118 C2H2 zinc finger proteins by AP-MS using GFP-tagged constructs in order to understand their role in transcription regulation.
ORGANISM(S): Homo sapiens (Human) 
2016-12-09 | PXD003431 | Pride
Protein protein interaction Regulation of transcription Transcription factors C2h2 zinc finger proteins 
TranscriptomicsSequence specificity is obtained from the majority of modular C2H2 zinc-finger arrays
C2H2 zinc fingers (C2H2-ZFs) are the most prevalent type of vertebrate DNA-binding domain, and typically appear in tandem arrays (ZFAs), with sequential C2H2-ZFs each contacting 3 (or more) sequential bases. C2H2-ZFs can be assembled in a modular fashion, providing one explanation for their remarkab...
ORGANISM(S): synthetic construct 
2010-12-02 | E-GEOD-25723 | biostudies-arrayexpress
TranscriptomicsMultiparameter functional diversity of human C2H2 zinc finger proteins [RNA-Seq]
C2H2 zinc finger proteins represent the largest and most enigmatic class of human transcription factors. Their C2H2 arrays are highly variable, indicating that most will have unique DNA binding motifs. However, most of the binding motifs have not been directly determined. We have determined the bind...
ORGANISM(S): Homo sapiens 
2016-10-25 | E-GEOD-76495 | biostudies-arrayexpress
TranscriptomicsEvaluation of DNA-binding preferences of C2H2 zinc finger domains by PBM
The largest and most diverse class of eukaryotic transcription factors contain Cys2-His2 zinc fingers (C2H2-ZFs), each of which typically binds a DNA nucleotide triplet within a larger binding site. Frequent recombination and diversification of their DNA-contacting residues suggests that these zinc...
ORGANISM(S): synthetic construct 
2015-02-17 | E-GEOD-52520 | biostudies-arrayexpress
TranscriptomicsHT-SELEX of recombinant C2H2 zinc finger domains of Sall4
To define the sequence preference of SALL4 C2H2 zinc finger domains, we performed SELEX coupled with high-throughput sequencing (HT-SELEX) using the purified SALL4 ZFC1, ZFC2 and ZFC4 domains combined with no protein control experiment.
ORGANISM(S): artificial sequences 
2021-01-05 | E-MTAB-9236 | biostudies-arrayexpress
GenomicsSelective Functions of Individual Zinc Fingers Within the DNA-Binding Domain of Ikaros (ChIP-seq: Thymocytes)
The C2H2 zinc finger is the most prevalent DNA-binding motif in the mammalian proteome, with DNA-binding domains usually containing more tandem fingers than are needed for stable sequence-specific DNA recognition. To examine the reason for the frequent presence of multiple zinc fingers, we generated...
ORGANISM(S): Mus musculus 
2013-09-08 | E-GEOD-33679 | biostudies-arrayexpress
TranscriptomicsSelective Functions of Individual Zinc Fingers Within the DNA-Binding Domain of Ikaros (RNA-seq: sorted DP thymocytes)
The C2H2 zinc finger is the most prevalent DNA-binding motif in the mammalian proteome, with DNA-binding domains usually containing more tandem fingers than are needed for stable sequence-specific DNA recognition. To examine the reason for the frequent presence of multiple zinc fingers, we generated...
ORGANISM(S): Mus musculus 
2013-09-08 | E-GEOD-47496 | biostudies-arrayexpress
TranscriptomicsLarge-scale characterization of DNA binding landscape of eukaryotic C2H2 zinc fingers
This SuperSeries is composed of the SubSeries listed below. Cys2-His2 zinc finger (C2H2-ZF) proteins represent the largest class of putative human transcription factors (TFs). However, it is unknown whether most C2H2-ZFs even bind DNA, or what sequences they bind. Using a combination of bacterial...
ORGANISM(S): synthetic construct 
2015-02-17 | E-GEOD-52523 | biostudies-arrayexpress
TranscriptomicsSelective Functions of Individual Zinc Fingers Within the DNA-Binding Domain of Ikaros (RNA-seq: sorted proB cell Hardy Fractions B and C+C')
The C2H2 zinc finger is the most prevalent DNA-binding motif in the mammalian proteome, with DNA-binding domains usually containing more tandem fingers than are needed for stable sequence-specific DNA recognition. To examine the reason for the frequent presence of multiple zinc fingers, we generated...
ORGANISM(S): Mus musculus 
2013-09-08 | E-GEOD-38842 | biostudies-arrayexpress
ProteomicsPBK/TOPK mediates Ikaros, Aiolos and CTCF displacement from mitotic chromosomes and alters chromatin accessibility at selected C2H2-zinc finger protein binding sites
PBK/TOPK is a mitotic kinase implicated in haematological and non-haematological cancers. Here we show that the key haemopoietic regulators Ikaros and Aiolos require PBK-mediated phosphorylation to dissociate from chromosomes in mitosis. Eviction of Ikaros is rapidly reversed by addition of the PBK-...
ORGANISM(S): Mus musculus (Mouse) 
2025-07-21 | PXD057894 | Pride
C2h2-zinc finger proteins Pbk/topk Chromosomes Mitosis Phosphorylation 
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