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TranscriptomicsRNA Sequencing Analysis of Mouse Bone Marrow Derived Dendritic Cells Transcriptomes During Maturation
Purpose:The purpose of this study is to detect activated or silenced genes during LPS-induced dendritic cell maturation. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse dendritic cells were generated from bone marrow cell...
ORGANISM(S): Mus musculus 
2015-03-04 | E-GEOD-60307 | biostudies-arrayexpress
TranscriptomicsSmall RNA Solexa sequencing of mouse hematopoietic progenitor and dendritic cell samples
We applied Illumina massively parallel signature sequencing to identify miRNomes in hematopoietic stem cells, bone morrow-derived immature DCs, mature DCs, and IL-10 and NO-producing regulatory DCs.The miRNomes of these DC subsets will contribute to investigate the significance of miRNAs in DC immun...
ORGANISM(S): Mus musculus 
2015-06-16 | E-GEOD-45882 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing Facilitates Quantitative Analysis of dendritic cell Transcriptomes during LPS response
Purpose:The purpose of this study is to detect activated or silenced genes during LPS-induced dendritic cell. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse dendritic cells were generated from bone marrow cells in RPMI-...
ORGANISM(S): Mus musculus 
2015-09-11 | E-GEOD-69256 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing Facilitates Quantitative Analysis of peritoneal macrophages Transcriptomes of Dnmt3a macrophage conditional knockout mouse
Purpose:The purpose of this study is to measure the gene expression profile in Dnmt3a conditional knockout macrophages. Methods:Dnmt3a conditional knockout macrophages mRNA profiles were generated by deep sequencing,using Illumina. Results: We mapped about 20 million sequence reads per sample to the...
ORGANISM(S): Mus musculus 
2016-05-30 | E-GEOD-74750 | biostudies-arrayexpress
TranscriptomicsSmall RNA Solexa sequencing of mouse CD11c+Ia high and CD11c+Ia low cells
We applied Illumina massively parallel signature sequencing to identify miRNomes in CD11c+Ia high and CD11c+Ia low cells.The miRNomes of these DC subsets will contribute to investigate the significance of miRNAs in DC immunobiology. Examination of miRNome in CD11c+Ia high and CD11c+Ia low cells . Al...
ORGANISM(S): Mus musculus 
2015-06-16 | E-GEOD-51574 | biostudies-arrayexpress
TranscriptomicsAn PWI- and RRM Motif-Containing Protein Is Critical for Pre-mRNA Splicing and ABA Responses in Arabidopsis
We used RNA-seq platform to determine role of a splicing factor RBM25 in regulation of gene expression and pre-mRNA splicing. We found that a loss-of-function allele of RBM25, rbm25-1, causes up- and down-regulation of a large number of genes. We further found that the rbm25-1 mutation results in d...
ORGANISM(S): Arabidopsis thaliana 
2015-10-28 | E-GEOD-66737 | biostudies-arrayexpress
ProteomicsQuantitative proteomics of Col and AtPABs mutant
To determine if the difference in AtPAB-binding efficiency affects protein levels, we performed the Tandem Mass Tag (TMT)-based quantitative proteomics on the two-week old seedlings of Col and the mut, respectively.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-10-11 | PXD014071 | Pride
Poly(a)-binding proteins  binding efficiency  translationa  arabidopsis 
ProteomicsA translational regulator MHZ9 modulates ethylene signaling in rice
In this project, we identified a novel RNA-binding protein, MHZ9. And we analyzed the potential proteins interacted with MHZ9 through immunoprecipitation-mass spectrometry (IP-MS). The N-terminal domain of MHZ9 (MHZ9-N) contains a putative RNA splicing and modification domain PRP4. To identify RNA b...
ORGANISM(S): Oryza sativa (Rice) 
2023-07-20 | PXD041240 | Pride
Q exactive mass spectrometer (thermo scientific) Gyf-domain Oryza_sativa Rna-crosslinked peptide 
UnknownRole of BMI1 in epithelial ovarian cancer: investigated via the CRISPR/Cas9 system and RNA sequencing.
Not available
S-EPMC5911954 | biostudies-literature
ProteomicsPhotoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatial-temporal resolution in live cells
Proximity labeling (PL) characterizes protein interactome in living cells. However, exogenous H2O2 required for engineered peroxidase, like APEX2, is cytotoxic, while biotin ligases-based PL have poor temporal resolution. Here, we showed that SOPP3, an engineered photosensitizer, could trigger APEX2...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD055708 | Pride
Proximity labeling Apex2 Sopp3 Protein-protein interaction Photosensitizer 
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