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ProteomicsQuantitative Native Proteomics by Capillary Zone Electrophoresis-Mass Spectrometry
Accurately measuring protein complex dynamics (i.e., composition and/or abundance changes) in cells is vital for advancing fundamental and translational research. In this work, we present a pilot study establishing capillary zone electrophoresis (CZE)-mass spectrometry (MS) based quantitative native...
ORGANISM(S): Escherichia coli 
2025-12-08 | PXD068962 | Pride
Capillary zone electrophoresis-mass spectrometry Quantitative native proteomics 
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of mouse brain integral membrane proteins
Mass spectrometry (MS)-based top-down characterization of integral membrane proteins (IMPs) is crucial for understanding their functions in biological processes. However, it is technically challenging due to their low solubility in typical MS-compatible buffers. In this work, for the first time, we ...
ORGANISM(S): Mus musculus (Mouse) 
2023-12-07 | PXD042298 | Pride
Top-down proteomics  integral membrane protein  proteoform  capillary zone electrophoresis-mass spectrometry  mouse brain 
ProteomicsA simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has become a valuable analytical technique in top-down proteomics (TDP) in recent years. CZE-MS/MS-based TDP typically employs separation capillaries with neutral coatings (i.e., linear polyacrylamide, LPA). However, issues related ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-15 | PXD051862 | Pride
Top-down proteomics  proteoform  capillary zone electrophoresis-mass spectrometry  covalent cationic coating  reproducibility  large proteoforms 
ProteomicsCapillary Zone Electrophoresis-High Field Asymmetric Ion Mobility Spectrometry-Tandem Mass Spectrometry for Top-down Characterization of Histone Proteoforms
Characterization of histone proteoforms with various post-translational modifications (PTMs) is critical for a better understanding of functions of histone proteoforms in epigenetic control of gene expression. Mass spectrometry (MS)-based top-down proteomics (TDP) is a valuable approach for delineat...
ORGANISM(S): Bos taurus (Bovine) 
2023-12-07 | PXD041357 | Pride
Histone Top-down proteomics High-field asymmetric waveform ion mobility spectrometry Post-translational modification Capillary zone electrophoresis 
MetabolomicsComparative characterization of the infant gut microbiome and their maternal lineage by a multi-omics approach

The human gut microbiome establishes and matures during infancy, and dysregulation at this stage may lead to pathologies later in life. We conducted a multi-omics study comprising three generations of family members to investigate the early development of the gut microbiota. Fecal samples from 20...

2024-03-07 | MTBLS7670 | MetaboLights
ProteomicsSolid phase microextraction-aided capillary zone electrophoresis-mass spectrometry: ready for bottom-up proteomics of single human cells
Capillary zone electrophoresis-mass spectrometry (CZE-MS) has been recognized as a valuable technique for proteomics of mass-limited biological samples (i.e., single cells) due to its high efficiency and high sensitivity for peptide separation and detection. However, its broad adoption for single ce...
ORGANISM(S): Homo sapiens (Human) 
2024-05-01 | PXD047627 | Pride
Hela Single cell proteomics Mass limited Solid phase microextraction Mass spectrometry Bottom-up proteomics 
ProteomicsLaser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain
Mass spectrometry (MS)-based spatially resolved top-down proteomics (TDP) of tissues is crucial for understanding the roles played by microenvironmental heterogeneity in the biological functions of organs and for discovering new proteoform biomarkers of diseases. There are few published spatially re...
ORGANISM(S): zebrafish metagenome 
2023-03-11 | PXD027741 | Pride
Spatially resolved top-down proteomics  capillary zone electrophoresis-tandem mass spectrometry  laser capture microdissection  zebrafish brain  proteoform  high sensitivity  neuropeptide hormone activity  motor activity 
ProteomicsStrong cation exchange-reversed phase liquid chromatography-capillary zone electrophoresis-tandem mass spectrometry platform with high peak capacity for deep bottom-up proteomics
Two-dimensional (2D) liquid chromatography (LC)-tandem mass spectrometry (MS/MS) are typically employed for deep bottom-up proteomics, and the state-of-the-art 2D-LC-MS/MS has approached over 8,000 protein identifications (IDs) from mammalian cell lines or tissues in 1-3 days of mass spectrometer ti...
ORGANISM(S): Mus musculus (Mouse) 
2018-02-27 | PXD008432 | Pride
Strong cation exchange  reversed-phase liquid chromatography  capillary zone electrophoresis  tandem mass spectrometry  bottom-up proteomics 
UnknownAnalysis of Proteins, Protein Complexes, and Organellar Proteomes Using Sheathless Capillary Zone Electrophoresis - Native Mass Spectrometry.
Not available
S-EPMC5709234 | biostudies-literature
ProteomicsSpatially-Resolved Top-Down Proteomics of Zebrafish Brain Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry
Understanding region-specific proteoform profiles in the brain is crucial for deciphering neural function and identifying potential therapeutic targets. Here, we present a comprehensive label-free quantitative top-down proteomics (TDP) analysis of distinct zebrafish brain regions using capillary zon...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2026-06-08 | PXD070925 | Pride
Proteoform Top-down proteomics Zebrafish brainregions 
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